The relationship between interphasic silver-stained proteins of the nucleolar organizer regions (Ag-NOR proteins) and cell replication rate has been studied in 13 established neuroblastoma cell lines. The quantity of Ag-NOR proteins was measured in silver-stained cells by means of an automated image analyzer. The results indicated that the amount of Ag-NOR proteins is strictly proportional to the proliferative activity of the cells. Cell lines with a difference of only 4 h in doubling time were characterized by a statistically significant difference in Ag-NOR protein amount. The Ag-NOR protein quantity can therefore be used as a parameter for evaluating the cell proliferation rate.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0014-4827(89)90371-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The value and significance of nucleolar organizer region proteins as markers of malignancy in breast cancer patients.
Saudi Med J
October 2024
From the Department of Clinical Laboratory Sciences (Salih, Dahlawi), College of Applied Medical Sciences, Taif University, Taif, from the Department of Medical Laboratory (Abdulgafor), King Faisal Special Hospital Research Center, Al-Madinah Al-Munawarah, and from the Department of Laboratory Medicine (Khalifa), Faculty of Applied Medical Science, University of Al Baha, Al Baha, Kingdom of Saudi Arabia.
Objectives: To assess argyrophilic nucleolar organizer regions (AgNORs) in 60 patients with primary breast carcinoma and evaluated their association with clinical prognostic parameters of breast cancer.
Methods: Argyrophilic nucleolar organizer regions were stained in paraffin sections of the tissues using Ploton's silver method. For each sample, the number of AgNORs within the nuclei of 100 tumor cells was counted.
Evaluation of the relationship between the expression of AgNOR and Ki67 with the recurrence rate in central granulomatous giant cell lesions: A case-control.
Clin Exp Dent Res
April 2024
Department of oral and maxillofacial pathology, School of Dentistry, Alborz University of Medical Science, Karaj, Iran.
Objectives: Giant cell granuloma is a local nonneoplastic lesion that is divided into two categories, based on its site of occurrence: Central and peripheral giant cell granuloma. Central giant cell granuloma is an intraosseous lesion that has a tendency to recure even in surgically treated cases. Several studies have proven that there is an association between different lesions clinical behavior and their histological features.
View Article and Find Full Text PDFA CNN-based approach for joint segmentation and quantification of nuclei and NORs in AgNOR-stained images.
Comput Methods Programs Biomed
December 2023
Instituto de Informática, Universidade Federal do Rio Grande do Sul, Av. Gonçalves, 9500, Porto Alegre, 91501-970, RS, Brazil. Electronic address:
Background And Objective: Oral cancer is the sixth most common kind of human cancer. Brush cytology for counting Argyrophilic Nucleolar Organizer Regions (AgNORs) can help early mouth cancer detection, lowering patient mortality. However, the manual counting of AgNORs still in use today is time-consuming, labor-intensive, and error-prone.
View Article and Find Full Text PDFRutin is one of the flavonoids found in fruits and vegetables. The PI3K/AKT/mTOR signaling pathway is critical for the life cycle at the cellular level. In current study, we purposed to demonstrate the antitumoral effect of rutin at different doses through the mTOR-signaling pathway and argyrophilic nucleolar regulatory region.
View Article and Find Full Text PDFUse of AgNOR staining to determine the effect of metoclopramide on neural tube development in early chick embryos.
Biotech Histochem
November 2023
Department of Anatomy, Medicine Faculty, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Nausea and vomiting during pregnancy are common problems and prolonged pharmacological treatment often is needed; however, the teratogenic effects of anti-emetic drugs on neural tube (NT) development are not clear. We investigated the effects of different doses of metoclopramide on NT development in 48 and 72 h chick embryos using an argyrophilic nucleolar organizing region (AgNOR) staining method. We used 150 fertile, specific pathogen-free eggs incubated for 28 h, then randomly divided into five equal groups: group A, sham control was administered 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!