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| http://dx.doi.org/10.1093/nar/17.17.7118 | DOI Listing |
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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders.
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December 2024
Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium.
Since the precursor frequency of naive T cells is extremely low, investigating the early steps of antigen-specific T cell activation is challenging. To overcome this detection problem, adoptive transfer of a cohort of T cells purified from T cell receptor (TCR) transgenic donors has been extensively used but is not readily available for emerging pathogens. Constructing TCR transgenic mice from T cell hybridomas is a labor-intensive and sometimes erratic process, since the best clones are selected based on antigen-induced CD69 upregulation or IL-2 production in vitro, and TCR chains are polymerase chain reaction (PCR)-cloned into expression vectors.
View Article and Find Full Text PDFAssociation between asymptomatic submicroscopic and microscopic malaria infections and anemia: A study in southern Benin.
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Université Paris Cité, IRD, MERIT, F-75006, Paris, France.
Introduction: Recently, efforts to eliminate malaria have shifted focus from symptomatic cases alone to include asymptomatic carriers, who are now recognized as significant contributors to the disease's transmission and control. This study examines the relationship between asymptomatic malaria infection and hemoglobin levels in Benin.
Methods: A cohort in Benin was enrolled and categorized into three age groups (under 5 years, 5-15 years, and over 15 years) for follow-up from August to November 2021.
First report of MDR virulent Pseudomonas aeruginosa in apparently healthy Japanese quail (Coturnix japonica) in Bangladesh.
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Department of Microbiology and Hygiene, Mymensingh, Bangladesh.
Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen associated conditions like septicaemia, respiratory disorders, and diarrhoea in poultry, particularly in Japanese quail (Coturnix japonica). The infection causes huge economical losses due to its high transmissibility, mortality and zoonotic potential.
View Article and Find Full Text PDFGenetic tracking of Crimean-Congo haemorrhagic orthonairovirus in Hyalomma population infesting cattle in Nigeria.
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Faculty of Veterinary Medicine, Department of Veterinary Microbiology, Arbovirology Unit, University of Ibadan, Ibadan, Nigeria.
Crimean-Congo haemorrhagic fever virus (CCHFV), a Biosafety level 4 pathogen transmitted by ticks, causes severe haemorrhagic diseases in humans but remains clinically silent in animals. Over the past forty years, Nigeria lacks comprehensive genetic data on CCHFV in livestock and ticks. This study aimed to identify and characterize CCHFV strains in cattle and their Hyalomma ticks, the primary vector, in Kwara State, Nigeria.
View Article and Find Full Text PDFEvaluation of the biocontrol potential of Episyrphus balteatus (Diptera: Syrphidae) on wheat aphids in northern China.
J Econ Entomol
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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Marmalada hoverfly, Episyrphus balteatus De Geer (Diptera: Syrphidae), is a cosmopolitan fly species providing pest control and pollination services. As wheat aphids cause significant losses to global wheat production, a systematic evaluation of the predatory potential and biocontrol service functions of E. balteatus in wheat ecosystems was undertaken.
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