Cigarette smoke has been shown to cause chronic inflammation of the lungs, eventually leading to chronic obstructive pulmonary disease (COPD). Additionally, recent studies have suggested that mesenchymal stem cells (MSCs) can mediate local inflammatory responses in the lungs. Thus, the aim of the present study was to test the effects of rat MSCs (rMSCs) on inflammation of the lungs and destructive pulmonary function induced by cigarette smoke in rats. Rats were exposed to cigarette smoke for 7 weeks. rMSCs were cultured in vitro and infused intratracheally into cigarette smoke-exposed rats. The total and differential cell counts in the bronchoalveolar lavage fluid (BALF), histological changes, pro-inflammatory cytokines, transforming growth factor-β1 (TGF-β1) expression, and pulmonary function were evaluated. Additionally, human peripheral blood mononuclear cells and human MSCs were cocultured in vitro to detect cytokines and TGF-β1 levels. We found that rMSC administration resulted in downregulation of pro-inflammatory cytokines in the lungs while increasing TGF-β1 expression, reducing total inflammatory cell numbers in the BALF, and improving pulmonary histopathology and airflow obstruction. Coculture revealed that human MSCs mediated an anti-inflammatory effect partly via upregulation of TGF-β1. These findings suggested that MSCs may have therapeutic potential in cigarette smoke-induced inflammation and airflow obstruction, partly via upregulation of TGF-β1.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3109/15412555.2014.898032 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of lung function in partially edentulous patients using spirometry: A cross-sectional study.
J Indian Prosthodont Soc
January 2025
Department of Dental Materials, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.
Aim: This study aimed to evaluate the effect of partially edentulous ()PED condition on lung function through spirometry tests and comparison of airflow rates between dentulous and PED subjects.
Settings And Design: The study design was a cross-sectional study conducted in the department of prosthodontics.
Materials And Methods: Twenty-eight dentulous and 28 PED patients with an age range of 25-50 years were included in the study.
Long-term Satisfaction with CO2 Laser Treatment for Moderate to Major Rhinophyma: A Single-centre Study.
Acta Derm Venereol
January 2025
Dermatology and Venereology Division, Department of Medicine (Solna), Karolinska Institutet, Stockholm, Sweden; Dermato-Venereology Clinic, Karolinska University Hospital, Stockholm, Sweden; Nordiska kliniken, Stockholm, Sweden, Sweden.
Rhinophyma, a severe manifestation of rosacea, predominantly affects Caucasian males aged 50-70 and is characterized by thickening and enlargement of the nasal skin. The condition can seriously both impact cosmetic appearance and obstruct nasal breathing. While its appearance is distinct, conditions such as basal cell carcinoma can mimic it, complicating diagnosis.
View Article and Find Full Text PDFContext-aware analysis enhances autoscoring accuracy of home sleep apnea testing.
J Clin Sleep Med
January 2025
Natural Interaction Lab, Thom Building, Department of Engineering, University of Oxford, Oxford, United Kingdom.
Study Objectives: Home sleep apnea testing based on peripheral arterial tonometry (P-HSAT) is increasingly being deployed because of its ability to test for multiple nights. However, P-HSATs do not have access to modalities such as airflow and cortical arousals and instead rely on alternative sources of information to detect respiratory events. This results in an a-priori performance disadvantage.
View Article and Find Full Text PDFmiRNAome-metabolome wide association study reveals effects of miRNA regulation in eosinophilia and airflow obstruction in childhood asthma.
EBioMedicine
December 2024
Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA. Electronic address:
Background: There are important inter-relationships between miRNAs and metabolites: alterations in miRNA expression can be induced by various metabolic stimuli, and miRNAs play a regulatory role in numerous cellular processes, impacting metabolism. While both specific miRNAs and metabolites have been identified for their role in childhood asthma, there has been no global assessment of the combined effect of miRNAs and the metabolome in childhood asthma.
Methods: We performed miRNAome-metabolome-wide association studies ('miR-metabo-WAS') in two childhood cohorts of asthma to evaluate the contemporaneous and persistent miRNA-metabolite associations: 1) Genetic Epidemiology of Asthma in Costa Rica Study (GACRS) (N = 1121); 2) the Childhood Asthma Management Program (CAMP) (N = 312 and N = 454).
Inhalation of patchouli essential oil alleviates airway inflammation in cigarette smoke-induced COPD mice.
Sci Rep
December 2024
State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, People's Republic of China.
Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide, characterized by persistent respiratory symptoms and airflow limitations resulting from small airway injury, bronchial wall thickening, and hypersecretion of mucus. Current pharmacological interventions are ineffective in reversing these airflow limitations; In our study, we investigated the potential role of patchouli essential oil (PEO) in the treatment of COPD and its underlying molecular mechanisms, both in vitro and in vivo. To establish a cigarette smoke-induced COPD mice model, we exposed the mice to cigarette smoke (CS) and administered nasal drip of lipopolysaccharides (LPS).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!