Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1126/science.1253616 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transfer of and increases 3D growth and invasiveness in recipient cancer cells.
Extracell Vesicles Circ Nucl Acids
July 2024
Laboratory of James G. Patton, Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Aim: Extracellular communication via the transfer of vesicles and nanoparticles is now recognized to play an important role in tumor microenvironment interactions. Cancer cells upregulate and secrete abundant levels of and that can alter gene expression in donor and recipient cells. In this study, we sought to identify targets of and and conclusively demonstrate that microRNAs (miRNAs) can be functionally transferred from donor to recipient cells.
View Article and Find Full Text PDFExamining the NEUROG2-lineage and associated-gene expression in human cortical organoids.
Development
December 2024
Sunnybrook Research Institute, Biological Sciences Platform, Hurvitz Brain Sciences Program, 2075 Bayview Ave, Toronto, ON, M4N 3M5, Canada.
Proneural genes are conserved drivers of neurogenesis across the animal kingdom. How their functions have adapted to guide human-specific neurodevelopmental features is poorly understood. Here, we mined transcriptomic data from human fetal cortices and generated from human embryonic stem cell (hESC)-derived cortical organoids (COs) to show that NEUROG1 and NEUROG2 are most highly expressed in basal neural progenitor cells, with pseudotime trajectory analyses indicating that NEUROG1-derived lineages predominate early and NEUROG2 lineages later.
View Article and Find Full Text PDFStructure and Methyl-lysine Binding Selectivity of the HUSH Complex Subunit MPP8.
J Mol Biol
December 2024
Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge CB2 0AW, UK. Electronic address:
The Human Silencing Hub (HUSH) guards the genome from the pathogenic effects of retroelement expression. Composed of MPP8, TASOR, and Periphilin-1, HUSH recognizes actively transcribed retrotransposed sequences by the presence of long (>1.5-kb) nascent transcripts without introns.
View Article and Find Full Text PDFArrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes.
Nat Biomed Eng
December 2024
Institute of Neuropathology, University of Zurich, Zurich, Switzerland.
Arrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libraries can be constructed for the genome-wide ablation (19,936 plasmids) of human protein-coding genes and for their activation and epigenetic silencing (22,442 plasmids), with each plasmid encoding an array of four non-overlapping sgRNAs designed to tolerate most human DNA polymorphisms.
View Article and Find Full Text PDFSalivary gland protective and antiinflammatory effects of genistein in Sjögren's syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha.
Cell Mol Biol Lett
December 2024
School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Medical University, Guangzhou, 510140, China.
Article Synopsis
- - The study investigates how genistein can potentially treat Sjögren's syndrome (SS) by targeting the genistein-estrogen receptor alpha (ERα) complex, which regulates the X-inactive specific transcript (Xist) and impacts ferroptosis through acyl-CoA synthetase long-chain family member 4 (ACSL4) in salivary gland epithelial cells (SGECs).
- - Researchers used nondiabetic obese (NOD)/LtJ mice and treated SGECs with Interferon-γ to evaluate changes in symptoms like dry mouth and to examine pathological lesions and molecular mechanisms underlying SS.
- - Results showed that genistein reduced SS symptoms by increasing Xist expression and decreasing Acsl
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!