Many high-throughput analytical platforms, from next-generation DNA sequencing to drug discovery, rely on beads as carriers of molecular diversity. Microfluidic systems are ideally suited to handle and analyze such bead libraries with high precision and at minute volume scales; however, the challenge of introducing bead suspensions into devices before they sediment usually confounds microfluidic handling and analysis. We developed a bead suspension hopper that exploits sedimentation to load beads into a microfluidic droplet generator. A suspension hopper continuously delivered synthesis resin beads (17 μm diameter, 112,000 over 2.67 h) functionalized with a photolabile linker and pepstatin A into picoliter-scale droplets of an HIV-1 protease activity assay to model ultraminiaturized compound screening. Likewise, trypsinogen template DNA-coated magnetic beads (2.8 μm diameter, 176,000 over 5.5 h) were loaded into droplets of an in vitro transcription/translation system to model a protein evolution experiment. The suspension hopper should effectively remove any barriers to using suspensions as sample inputs, paving the way for microfluidic automation to replace robotic library distribution.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029372 | PMC |
| http://dx.doi.org/10.1021/ac500693r | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of topical florfenicol, terbinafine and mometasone furoate ear drops on adrenocortical function in healthy small breed dogs with bilateral otitis externa.
Vet Dermatol
June 2022
Office of Information and Technology, University of Tennessee, Knoxville, TN, 37996, USA.
Background: Testing for hyperadrenocorticism is commonly pursued in adult dogs with dermatological disease, and adrenocortical suppression has been well-documented following the use of topical corticosteroids in otic preparations. An otic suspension that contains florfenicol, terbinafine and mometasone furoate, and lasts for 30 days after a single application, frequently is used to treat canine otitis externa (OE). This medication was shown to cause adrenocortical suppression on Day (D)2 postadministration and two weeks after two applications two weeks apart.
View Article and Find Full Text PDFStrategic placement of an obstacle suppresses droplet break up in the hopper flow of a microfluidic soft crystal.
Proc Natl Acad Sci U S A
May 2021
Department of Mechanical Engineering, Stanford University, Stanford, CA 94305
When granular materials, colloidal suspensions, and even animals and crowds exit through a narrow outlet, clogs can form spontaneously when multiple particles or entities attempt to exit simultaneously, thereby obstructing the outlet and ultimately halting the flow. Counterintuitively, the presence of an obstacle upstream of the outlet has been found to suppress clog formation. For soft particles such as emulsion drops, clogging has not been observed in the fast flow limit due to their deformability and vanishing interparticle friction.
View Article and Find Full Text PDFhνSABR: Photochemical Dose-Response Bead Screening in Droplets.
Anal Chem
March 2016
Department of Chemistry and ‡Doctoral Program in Chemical and Biological Sciences, The Scripps Research Institute, Jupiter, Florida 33458, United States.
With the potential for each droplet to act as a unique reaction vessel, droplet microfluidics is a powerful tool for high-throughput discovery. Any attempt at compound screening miniaturization must address the significant scaling inefficiencies associated with library handling and distribution. Eschewing microplate-based compound collections for one-bead-one-compound (OBOC) combinatorial libraries, we have developed hνSABR (Light-Induced and -Graduated High-Throughput Screening After Bead Release), a microfluidic architecture that integrates a suspension hopper for compound library bead introduction, droplet generation, microfabricated waveguides to deliver UV light to the droplet flow for photochemical compound dosing, incubation, and laser-induced fluorescence for assay readout.
View Article and Find Full Text PDF3D CFD simulations of trailing suction hopper dredger plume mixing: a parameter study of near-field conditions influencing the suspended sediment source flux.
Mar Pollut Bull
November 2014
Section of Dredging Engineering, Faculty 3mE, Delft University of Technology, Mekelweg 2, 2628 CD Delft, The Netherlands. Electronic address:
Frequency, duration and intensity of stresses like turbidity and sedimentation caused by dredging must be known to determine the environmental impact of dredging projects. These stresses depend on the amount of sediment spill from a dredger and on how much of this spill still is in suspension near environmentally sensitive areas. Near-field mixing close to a dredger influences the deposition behaviour of the sediment spill.
View Article and Find Full Text PDFMicrofluidic bead suspension hopper.
Anal Chem
May 2014
Department of Chemistry, The Scripps Research Institute, Jupiter, Florida 33458, United States.
Many high-throughput analytical platforms, from next-generation DNA sequencing to drug discovery, rely on beads as carriers of molecular diversity. Microfluidic systems are ideally suited to handle and analyze such bead libraries with high precision and at minute volume scales; however, the challenge of introducing bead suspensions into devices before they sediment usually confounds microfluidic handling and analysis. We developed a bead suspension hopper that exploits sedimentation to load beads into a microfluidic droplet generator.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!