AI Article Synopsis
- The study utilized DNA barcoding to accurately identify Radix et Rhizoma Clematidis and distinguish it from its adulterants.
- Researchers amplified and sequenced the psbA-trnH region and compared it with 284 sequences from GenBank, evaluating five different barcodes for identification efficiency.
- Results indicated that psbA-trnH outperformed the other barcodes in terms of identification efficiency, genetic divergence, and analysis, making it the preferred choice for this type of identification.
Article Abstract
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
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