AI Article Synopsis
- A family of fluorescent probes has been developed that responds to pH changes, based on a specific chemical structure known to be a strong electron acceptor.
- Structural variations in the probes can adjust their acidity, represented by a pK(a) range from 4.8 to 8.6.
- Significant changes in fluorescence intensity were observed with different pH levels, and the most effective probes for near-neutral pH were utilized for imaging pH levels inside cells.
Article Abstract
A family of fluorescent push-pull pH-responsive probes based on 2-dicyanomethylidene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran as a strong electron acceptor group is described. Small structural variations allow obtaining pK(a) ranging from 4.8 to 8.6, underlining the role of the substituent in modulating the acidic properties. Remarkable changes in the optical properties (in particular the fluorescence intensity ratios) were observed as a function of pH. The most interesting probes with pK(a) close to neutrality were used for ratiometric imaging of intracellular pH.
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| http://dx.doi.org/10.1039/c4ob00147h | DOI Listing |
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