There is interest currently in the AgNOR technique, for NOR-associated proteins. In some cases, this technique enables the distinction to be made between certain grades of malignancy and may enable prognostic assessment. This paper attempts to suggest a standardized means for the enumeration of NORs in histological sections. Attention should be paid to rigorous technique and careful resolution of intranucleolar AgNOR dots. The timing of the reaction and fixation methods are also most important.

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http://dx.doi.org/10.1002/path.1711580303DOI Listing

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