A biocatalytic cascade with several output signals--towards biosensors with different levels of confidence.

Anal Bioanal Chem

Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, 13699-5810, USA.

Published: May 2014

AI Article Synopsis

  • Developed a biocatalytic cascade using enzyme reactions activated by biomolecular signals that output results after each step.
  • This system mimics concatenated AND logic gates, producing optically readable signals throughout the process.
  • Potential applications include enhanced bioanalyses and data interpretation for improved medical diagnostics.

Article Abstract

The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical diagnostics.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072206PMC
http://dx.doi.org/10.1007/s00216-014-7789-5DOI Listing

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