AI Article Synopsis
- A new, efficient LC-MS/MS method was developed for measuring melatonin levels in milk.
- The method involves extracting samples using dichloromethane and purifying them with a solid-phase extraction cartridge before analysis.
- Results showed the method is accurate (92.2% accuracy), precise (1.55% mean precision), and has a strong linear calibration range, making it suitable for examining melatonin in both market and human milk.
Article Abstract
A rapid, reliable and sensitive LC-MS/MS method for the determination of melatonin in milk was developed and validated. Sample was extracted with dichloromethane and cleaned by passing through Chem Elut solid phase extraction cartridge. The solvent was evaporated to dryness, reconstituted with methanol and analysed by LC-MS/MS on Agilent zorbax Eclipse XDB C-18 rapid resolution analytical column. The analytical procedure was found to be accurate, precise and linear. The method accuracy was 92.2 % (range 90.06-94.58) and the mean precision was 1.55 % and the calibration was linear for 1 to 150 pg mL(-1) (R(2) > 0.99), the lowest limit of quantification (LLOQ) was 1 pg mL(-1). 7-D Melatonin (7-DM) was used as an internal standard. This method was proved to be a promising method for the determination of melatonin for market milk and human milk samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981994 | PMC |
| http://dx.doi.org/10.1007/s13197-013-1221-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!