A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤ 1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4042775 | PMC |
| http://dx.doi.org/10.1128/JCM.03389-13 | DOI Listing |
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