AI Article Synopsis
- The study describes a method to synthesize 1-(13)C-phosphoenolpyruvate-d2, which is a precursor for enhancing the polarization of 1-(13)C-phospholactate-d2 using parahydrogen-induced polarization (PHIP).
- The (13)C nuclear spin polarization of 1-(13)C-phospholactate-d2 was greatly increased, achieving levels over 30 million times higher in water and reaching a polarization level above 15.6%.
- This compound demonstrates a long hyperpolarization lifetime (58 ± 4 seconds) and significant production quantities, suggesting its potential as a contrast agent for metabolic imaging and other applications.
Article Abstract
The synthetic protocol for preparation of 1-(13)C-phosphoenolpyruvate-d2, precursor for parahydrogen-induced polarization (PHIP) of 1-(13)C-phospholactate-d2, is reported. (13)C nuclear spin polarization of 1-(13)C-phospholactate-d2 was increased by >30,000,000-fold (5.75 mT) in water. The reported (13)C polarization level approaching unity (>15.6%), long lifetime of (13)C hyperpolarized 1-(13)C-phospholactate-d2 (58 ± 4 s versus 36 ± 2 s for nondeuterated form at 47.5 mT), and large production quantities (52 μmoles in 3 mL) in aqueous medium make this compound useful as a potential contrast agent for the molecular imaging of metabolism and other applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063326 | PMC |
| http://dx.doi.org/10.1021/ac500952z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays.
STAR Protoc
January 2025
Department of Molecular Medicine, University of Pavia, Pavia, Italy. Electronic address:
Voltage-dependent anion channel 1 (VDAC1) is a key protein in cellular metabolism and apoptosis. Here, we present a protocol to express and purify milligram amounts of recombinant VDAC1 in Escherichia coli. We detail steps for a fluorescence polarization-based high-throughput screening assay using NADH displacement, along with procedures for thermostability, fluorescence polarization, and X-ray crystallography.
View Article and Find Full Text PDFDipyridamole Attenuates Experimental Periodontitis by Regulating M1 Macrophage Polarization via PKA/PKG Pathways.
J Periodontal Res
January 2025
Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Aim: Periodontitis is a chronic inflammatory disease initiated by dysbiosis of the local microbial community. As a non-specific phosphodiesterase inhibitor, dipyridamole features anti-oxidant and anti-inflammatory properties. This study aimed to investigate the effects of dipyridamole in an experimental rat model of periodontitis.
View Article and Find Full Text PDFAntioxidant, antiproliferative and anti-inflammatory activities of squirting cucumber (Ecballium elaterium L).
Pak J Pharm Sci
January 2025
Laboratory of Aromatic and Medicinal Plants, Borj Cedria Biotechnology Center, Hammam-Lif, Tunisia.
Plants constitute a source of natural phytochemical components which are widely known for their potential biological activities. This work concerned a study of the antioxidant, anticancer and anti-inflammatory activities of squirting cucumber (Ecballium elaterium L.) parts (flowers, fruits, leaves and stems) using different solvent extracts (cyclohexane, dichloromethane, ethyl acetate, methanol and water).
View Article and Find Full Text PDFFerroptosis-related genes participate in the microglia-induced neuroinflammation of spinal cord injury via NF-κB signaling: evidence from integrated single-cell and spatial transcriptomic analysis.
J Transl Med
January 2025
Division of Spine, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, Tongji University School of Medicine, Shanghai, 200065, China.
Background: Ferroptosis and immune responses are critical pathological events in spinal cord injury (SCI), whereas relative molecular and cellular mechanisms remain unclear.
Methods: Micro-array datasets (GSE45006, GSE69334), RNA sequencing (RNA-seq) dataset (GSE151371), spatial transcriptome datasets (GSE214349, GSE184369), and single cell RNA sequencing (scRNA-seq) datasets (GSE162610, GSE226286) were available from the Gene Expression Omnibus (GEO) database. Through weighted gene co-expression network analysis and differential expression analysis in GSE45006, we identified differentially expressed time- and immune-related genes (DETIRGs) associated with chronic SCI and differentially expressed ferroptosis- and immune-related genes (DEFIRGs), which were validated in GSE151371.
Time-varying 3D optical torque via a single beam.
Nat Commun
January 2025
Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, China.
The spin angular momentum (SAM) plays a significant role in light-matter interactions. It is well known that light carrying SAM can exert optical torques on micro-objects and drive rotations, but 3D rotation around an arbitrary axis remains challenging. Here, we demonstrate full control of the 3D optical torque acting on a trapped microparticle by tailoring the vectorial SAM transfer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!