Most Saccharomyces spp. cannot degrade or ferment dextrin, which is the second most abundant carbohydrate in wort for commercial beer production. Dextrin-degrading brewer's bottom and top yeasts expressing the glucoamylase gene (GAM1) from Debaryomyces occidentalis were developed to produce low-carbohydrate (calorie) beers. GAM1 was constitutively expressed in brewer's yeasts using a rDNA-integration system that contained yeast CUP1 gene coding for copper resistance as a selective marker. The recombinants secreted active glucoamylase, displaying both α-1,4- and α-1,6-debranching activities, that degraded dextrin and isomaltose and consequently grew using them as sole carbon source. One of the recombinant strains expressing GAM1 hydrolyzed 96 % of 2 % (w/v) dextrin and 98 % of 2 % (w/v) isomaltose within 5 days of growth. Growth, substrate assimilation, and enzyme activity of these strains were characterized.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10529-014-1530-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spent Brewer's Yeast Lysis Enables a Approach in the Beer Industry.
Int J Mol Sci
November 2024
Bioproducts Group, Bioresources Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, Spl. Independentei No. 202, Sector 6, 060021 Bucharest, Romania.
Yeasts have emerged as an important resource of bioactive compounds, proteins and peptides, polysaccharides and oligosaccharides, vitamin B, and polyphenols. Hundreds of thousands of tons of spent brewer's yeast with great biological value are produced globally by breweries every year. Hence, streamlining the practical application processes of the bioactive compounds recovered could close a loop in an important bioeconomy value-chain.
View Article and Find Full Text PDFAntimicrobial and Antifungal Action of Biogenic Silver Nanoparticles in Combination with Antibiotics and Fungicides Against Opportunistic Bacteria and Yeast.
Int J Mol Sci
November 2024
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Str. 18, 420008 Kazan, Russia.
The development of multidrug resistance by pathogenic bacteria and yeast is a significant medical problem that needs to be addressed. One possible answer could be the combined use of antibiotics and silver nanoparticles, which have different mechanisms of antimicrobial action. In the same way, these nanoparticles can be combined with antifungal agents.
View Article and Find Full Text PDFPollens of Fabaceae and Poaceae Alone or Combined with Honey Solution? Suitable Diet for Adults of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae).
Neotrop Entomol
December 2024
Programa de Pós-Graduação em Fitotecnia, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil.
Article Synopsis
- Adult Chrysoperla externa rely primarily on pollen for protein, and various pollen types affect their biology differently.
- A study compared the performance of C. externa fed different pollen diets against a standard diet (brewer's yeast + honey) and found some pollen diets supported egg production and longevity similar to the standard diet.
- Interestingly, mixtures of pollen from the Fabaceae and Poaceae families did not significantly outperform individual pollen types in terms of enhancing adult performance, challenging initial expectations.
Targeted Study of the Effect of Yeast Strain on Volatile Compounds Produced in Sorghum Beer.
Foods
November 2024
Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA.
An increase in the consumer demand and the availability of gluten-free products has led to several brewers investigating brewing with grains other than barley. The primary grain of choice has been sorghum. These new gluten-free beers have a unique flavor and aroma, which previous research has shown is the result of differences in concentration for key chemical compounds, including ethyl butyrate, butyl acetate, isoamyl acetate, ethyl caproate, hexyl acetate, 1-octanol, nonanal, ethyl octanoate, and ethyl decanoate.
View Article and Find Full Text PDFUsing Spent Brewer's Yeast to Encapsulate and Enhance the Bioavailability of Sonochemically Nanostructured Curcumin.
Int J Food Sci
November 2024
Faculty of Biotechnologies (BioTech), ITMO University, Kronverksky Prospekt, 49, lit. A, Saint Petersburg 197101, Russia.
This study is aimed at investigating the possibility of using spent cells of brewer's yeast to encapsulate the plant antioxidant curcumin and the effect of such an approach on the bioavailability of BAS in an digestion model. Spent brewer's yeast is a significant volume organic waste that is difficult to dispose of, which makes additional options for its use very promising. Encapsulation of curcumin into spent yeast cells was carried out in a nanostructured manner.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!