A tissue-engineered oesophageal scaffold could be very useful for the treatment of pediatric and adult patients with benign or malignant diseases such as carcinomas, trauma or congenital malformations. Here we decellularize rat oesophagi inside a perfusion bioreactor to create biocompatible biological rat scaffolds that mimic native architecture, resist mechanical stress and induce angiogenesis. Seeded allogeneic mesenchymal stromal cells spontaneously differentiate (proven by gene-, protein and functional evaluations) into epithelial- and muscle-like cells. The reseeded scaffolds are used to orthotopically replace the entire cervical oesophagus in immunocompetent rats. All animals survive the 14-day study period, with patent and functional grafts, and gain significantly more weight than sham-operated animals. Explanted grafts show regeneration of all the major cell and tissue components of the oesophagus including functional epithelium, muscle fibres, nerves and vasculature. We consider the presented tissue-engineered oesophageal scaffolds a significant step towards the clinical application of bioengineered oesophagi.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354271 | PMC |
| http://dx.doi.org/10.1038/ncomms4562 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical translation of tissue-engineered oesophageal grafts: are patients ready for us?
Pediatr Surg Int
November 2024
Stem Cell and Regenerative Medicine Section, Developmental Biology and Cancer Research and Teaching Department, Zayed Centre for Research Into Rare Disease in Children, UCL Great Ormond Street Institute of Child Health, London, UK.
Purpose: We sought to engage with expert patient/carers to understand attitudes towards use of tissue engineering (TE) for long-gap oesophageal atresia (OA).
Methods: An in-person engagement event for 70 patients/parents was held by the OA patient group, TOFS. Attitudes towards TE were assessed before and after a talk on use of TE oesophagi in a pre-clinical OA model.
Circumferential Esophageal Reconstruction Using a Tissue-engineered Decellularized Tunica Vaginalis Graft in a Rabbit Model.
J Pediatr Surg
August 2024
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Background: Pediatric surgeons have faced esophageal reconstruction challenges for decades owing to a variety of congenital and acquired conditions. This work aimed to introduce a reproducible and efficient approach for creating tissue-engineered esophageal tissue using bone marrow mesenchymal stem cells (BMSCs) cultured in preconditioned mediums seeded on a sheep decellularized tunica vaginalis (DTV) scaffold for partial reconstruction of a rabbit's esophagus.
Methods: DTV was performed using SDS and Triton X-100 solutions.
A laboratory X-ray imaging system with a setup that closely resembles commercial micro-CT systems with a fixed source-to-detector distance of ∼90 cm is investigated for single distance propagation-based phase-contrast imaging and computed tomography (CT). The system had a constant source-to-detector distance, and the sample positions were optimized. Initially, a PTFE wire was imaged, both in 2D and 3D, to characterize fringe contrast and spatial resolution for different X-ray source settings and source-to-sample distances.
View Article and Find Full Text PDFThe occurrence and development mechanisms of esophageal stricture: state of the art review.
J Transl Med
January 2024
Health Science Center, Ningbo University, Ningbo, 315211, People's Republic of China.
Article Synopsis
- Esophageal strictures are a major health issue that affect quality of life and pose treatment challenges due to their tendency to recur after procedures like endoscopic submucosal dissection (ESD) or endoscopic mucosal resection (EMR).
- Current therapies mainly relieve symptoms but do not target the underlying causes, which involve factors like fibroblast overactivity and chronic inflammation.
- Effective treatment relies on understanding the role of the esophageal epithelial barrier's dysfunction in stricture formation, with potential solutions including tissue-engineered cell patches to promote healing and prevent recurrence.
Dragging 3D printing technique controls pore sizes of tissue engineered blood vessels to induce spontaneous cellular assembly.
Bioact Mater
January 2024
Department of Mechanical and Design Engineering, Wonkwang University, 54538, Iksan, Republic of Korea.
To date, several off-the-shelf products such as artificial blood vessel grafts have been reported and clinically tested for small diameter vessel (SDV) replacement. However, conventional artificial blood vessel grafts lack endothelium and, thus, are not ideal for SDV transplantation as they can cause thrombosis. In addition, a successful artificial blood vessel graft for SDV must have sufficient mechanical properties to withstand various external stresses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!