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Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa. | LitMetric

Glycosylated yellow laccases of the basidiomycete Stropharia aeruginosa.

Enzyme Microb Technol

Institute of Ageing and Chronic Disease, Faculty of Health & Life Sciences, Department of Musculoskeletal Biology, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, United Kingdom. Electronic address:

Published: May 2014

AI Article Synopsis

  • This study focuses on the identification and purification of two glycosylated yellow laccase proteins (Yel1p and Yel3p) from the fungus Stropharia aeruginosa.
  • Both proteins are monomeric, N-glycosylated, and have a molecular weight of about 55kDa, but they lack the characteristic blue laccase absorption band at 612nm.
  • The research involved transcriptome sequencing and inverse-PCR to obtain cDNA sequences, particularly linking one to Yel1p, and it explores the unique properties of these yellow laccases compared to their blue counterparts based on biochemical and spectral analyses.

Article Abstract

Here we describe the identification, purification and characterisation of glycosylated yellow laccase proteins from the basidiomycete fungus Stropharia aeruginosa. Biochemical characterisation of two yellow laccases, Yel1p and Yel3p, show that they are both secreted, monomeric, N-glycosylated proteins of molecular weight around 55kDa with substrate specificities typical of laccases, but lacking the absorption band at 612nm typical of the blue laccase proteins. Low coverage, high throughput 454 transcriptome sequencing in combination with inverse-PCR was used to identify cDNA sequences. One of the cDNA sequences has been assigned to the Yel1p protein on the basis of identity between the translated protein sequence and the peptide data from the purified protein, and the full length gene sequence has been obtained. Biochemical properties, substrate specificities and protein sequence data have been used to discuss the unusual spectroscopic properties of S. aeruginosa proteins in the context of recent theories about the differences between yellow and blue laccases.

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Source
http://dx.doi.org/10.1016/j.enzmictec.2014.02.003DOI Listing

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