Evidence for considerable stabilization of doped bovine serum albumin (BSA) molecules upon adsorption on gold surfaces is provided. This is compared to the surface-induced conformational changes of the bare BSA and its corresponding monolayer. The BSA unfolding phenomenon is correlated with dehydration, which in turn enables improved monolayer coverage. The stabilization mechanism is found to be partially controllable via nanodoping of the BSA molecules, upon which the dehydration process is suppressed and molecular rigidity can be varied. Our experimental data and calculations further point to the intermixing of structural characteristics and inherent molecular properties in studies of biological monolayers.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja410754t | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Shape-responsive host-guest chemistry: metal-free tetracationic porphyrin nonplanarity promoted by clay mineral interactions assessed by theoretical simulations.
Dalton Trans
January 2025
Departamento de Física dos Materiais e Mecânica, Instituto de Física, Universidade de São Paulo, C. P. 66318, São Paulo, SP, 05508-090, Brazil.
Distortions in the porphyrin core from planarity can trigger a unique structure-property relationship, imparting its basicity, chemical stability, redox potential, and excited-state energetics, among other properties. The colour change promoted by such distortion is signed by red shifts in its electronic absorption spectra. The adsorption of guest -substituted free-base porphyrin species onto inorganic hosts, such as clay minerals (layered aluminium or magnesium silicates), is known to further promote colour changes.
View Article and Find Full Text PDFAssessing the interaction between the N-terminal region of the membrane protein magnesium transporter A and a lipid bilayer.
J Colloid Interface Sci
December 2024
Division of Computational Chemistry, Department of Chemistry, Lund University, P.O. Box 124, Lund, 22100, Sweden. Electronic address:
Unlabelled: This study investigates the interaction of KEIF, the intrinsically disordered N-terminal region of the magnesium transporter MgtA, with lipid bilayers mimicking cell membranes. Combining experimental techniques such as neutron reflectometry (NR), quartz-crystal microbalance with dissipation monitoring (QCM-D), synchrotron radiation circular dichroism (SRCD), and oriented circular dichroism (OCD), with molecular dynamics (MD) simulations, we characterized KEIF's adsorption behavior.
Hypothesis: KEIF undergoes conformational changes upon interacting with lipid bilayers, potentially influencing MgtA's function within the plasma membrane.
Roughhousing with Ions: Surface-Induced Dissociation and Electron Capture Dissociation as Diagnostics of Q-Cyclic IMS-TOF Instrument Tuning Gentleness.
J Am Soc Mass Spectrom
January 2025
Native MS Guided Structural Biology Center, The Ohio State University, Columbus, Ohio 43210, United States.
Article Synopsis
- Native mass spectrometry characterizes biomolecular features, but ionization tuning can distort the protein complex's structure, affecting experimental results.
- Surface-induced dissociation (SID) helps determine the native-like topology of protein complexes by causing them to dissociate at their weakest interfaces, but precedes changes in the quaternary structure can alter SID results.
- Electron-capture dissociation (ECD) complements SID by confirming rearranged subunit interfaces after in-source activation, emphasizing the importance of using standard protein complexes when calibrating instruments for accurate native mass spectrometry.
Conformational Dynamics of Hemoglobin in Solution and the Gas Phase Elucidated by Mass Spectrometry.
Anal Chem
December 2024
Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich CH-8093, Switzerland.
Solution and gas-phase measurements can provide valuable insights into biomolecular conformational dynamics. By comparing the data from such experiments, it is possible to elucidate the nature of the interactions governing a biomolecule's stability. Here, we measured human, bovine, and porcine hemoglobin stability in solution and the gas phase using collision-induced dissociation, collision-induced unfolding, surface-induced dissociation, and temperature-controlled nanoelectrospray mass spectrometry.
View Article and Find Full Text PDFConformational Switching of a Nano-Size Urea-Bridged Zn(II)Porphyrin Dimer by External Stimuli.
Chemistry
December 2024
Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
For the first time, explicit stabilization of all the three conformers, viz. (cis,cis), (cis,trans) and (trans,trans), of a 'nano-sized' highly-flexible urea-bridged Zn(II)porphyrin dimer have been achieved via careful manipulations of external stimuli such as solvent dielectrics, temperature, anionic interactions, axial ligation and surface-induced stabilization. The conformers differ widely in their structures, chemical and photophysical properties and thus have vast potential applicability.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!