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Simultaneous determination of cortisol and cortisone from human serum by liquid chromatography-tandem mass spectrometry. | LitMetric

AI Article Synopsis

  • - A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to measure cortisol and cortisone levels in healthy adults' serum, showing high sensitivity and precision.
  • - The method demonstrated accuracy rates between 89.2% and 102.5% and was capable of detecting cortisol and cortisone at very low concentrations (as low as 0.2 ng/mL for cortisol).
  • - Results indicated that average cortisol levels were significantly higher than cortisone levels in the samples tested, suggesting the method could aid in diagnosing adrenal diseases and evaluating specific enzyme activity in clinical settings.

Article Abstract

A fast, sensitive, and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was validated and then the levels of cortisol and cortisone from sera of healthy adults were determined by the LC-MS/MS method. One hundred μ L of serum sample was directly extracted by adding 2 mL ethyl acetate, followed by chromatographic separation on a C18 column with a mobile phase consisting of 5 mM ammonium acetate and methanol (25 : 75, v/v). The precision, accuracy, and average recovery of the method were 1.5-5.3%, 95.4-102.5%, and 96.4% for cortisol, and 1.9-6.0%, 89.2-98.8%, and 79.9% for cortisone, respectively. The method was linear from 1.0 to 500.0 ng/mL (r(2) = 0.999) for cortisol and 2.5 to 100.0 ng/mL (r(2) = 0.998) for cortisone. The limits of detection (LOD) and quantification (LOQ) were 0.2 and 1.0 ng/mL for cortisol, and 1.0 and 2.5 ng/mL for cortisone, respectively. The average cortisol concentration (133.9 ± 63.7 ng/mL) of samples collected between 9:00 and 11:00 a.m. was higher approximately 4.4 times than that of cortisone (30.5 ± 10.7 ng/mL) (P < 0.0001). The average cortisone/cortisol ratio was 0.225. Therefore, the LC-MS/MS method may be useful for the diagnosis of some adrenal diseases and the assessment of 11 β -hydroxysteroid dehydrogenase (11 β -HSD) activity in clinical laboratories.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963115PMC
http://dx.doi.org/10.1155/2014/787483DOI Listing

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