Application of pollination of opened ovaries to obtain L. × L. hybrids.

This study presents the results of experiments concerning: (1) interspecific hybridization of × via application of placental pollination and (2) embryological analysis of the process of resynthesis of . In order to overcome certain stigma/style barriers, pollen was placed on an opened ovary (with style removed). Pollinated ovaries were cultured on Murashige and Skoog (MS) medium. After 24-d culture, the developing embryos were isolated from immature seeds and transferred onto MS medium supplemented with 0.47 μM kinetin, 0.49 μM 1-naphthaleneacetic acid, and 10% (/) coconut water. When the embryos had turned green, they were immediately placed onto MS medium with 100 μM kinetin. After development of the seedling, plantlets were transferred to soil. Chromosome doubling was achieved after another week. Cytometric analysis of nuclear DNA confirmed the hybrid nature of the plants. Resynthesis of can be performed through interspecific hybridization of × followed by embryo rescue and genome doubling.