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Iron-sulfur cluster redox chemistry and dimer dissociation in the outer mitochondrial membrane protein, mitoNEET.
J Biol Inorg Chem
December 2024
Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH, USA.
The outer mitochondrial membrane protein known as mitoNEET was discovered when it was labeled by a photoaffinity derivative of the anti-diabetes medication, pioglitazone. The biological role for mitoNEET and its specific mechanism for achieving this remains an active subject for research. There is accumulating evidence suggesting that mitoNEET could be a component of mitochondrial FeS cofactor biogenesis.
View Article and Find Full Text PDFPreservative effects and mechanism of condensed tannins from Cercis chinensis Bunge leaves on fresh-cut lotus root.
J Food Sci
December 2024
School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, China.
This study aimed to investigate the potential of condensed tannins isolated from Cercis chinensis Bunge leaves as natural preservatives for fruits and vegetables. The research demonstrated that C. chinensis leaves condensed tannins (CLCT) significantly delay the browning process and reduce nutritional loss in fresh-cut lotus roots.
View Article and Find Full Text PDFUPLC-PDA-ESI-MS based chemometric analysis for solvent polarity effect evaluation on phytochemical compounds and antioxidant activity in habanero pepper (Capsicum chinense Jacq) fruit extract.
J Food Sci
December 2024
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ) Unidad Sureste, Tablaje Catastral Km 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de, Yucatán, Mexico.
The effect of solvents with different polarities on the recovery of phytochemicals (carotenoids, capsaicinoids, and phenolic compounds) from habanero pepper (Capsicum chinense) and their association with antioxidant activity (ABTS and DPPH) was evaluated through Ultra-Performance-Liquid Chromatography coupled with a Photodiode Array Detector and a Electrospray Ionization Mass Spectrometry (UPLC-PDA-ESI-MS)-based chemometric analysis, including linear correlation, multiple linear regression, and principal component analysis (PCA). The solvent polarity scale was established according to solvent dielectric constants (ɛ). Color variation (ΔE) was used to determine the presence of carotenoids, with the highest ΔE obtained using low-polarity solvents (hexane and ethyl acetate).
View Article and Find Full Text PDFEarly detection of bacterial pneumonia by characteristic induced odor signatures.
BMC Infect Dis
December 2024
Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zürich, Zurich, 8097, Switzerland.
Introduction: The ability to detect pathogenic bacteria before the onsets of severe respiratory symptoms and to differentiate bacterial infection allows to improve patient-tailored treatment leading to a significant reduction in illness severity, comorbidity as well as antibiotic resistance. As such, this study refines the application of the non-invasive Secondary Electrospray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) methodology for real-time and early detection of human respiratory bacterial pathogens in the respiratory tract of a mouse infection model.
Methods: A real-time analysis of changes in volatile metabolites excreted by mice undergoing a lung infection by Staphylococcus aureus or Streptococcus pneumoniae were evaluated using a SESI-HRMS instrument.
Brefeldin A-A Major Pathogenic Factor of Peanut Pod Rot from .
Toxins (Basel)
December 2024
Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
is the main pathogen of peanut pod rot in China. To investigate the type of toxin and its pathogenic mechanism, a macrolide, brefeldin A, was isolated. The structure of the compound was identified by 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS).
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