The preparation and application of a stop and go extraction tip (StageTip) used for pre-purification of sample solutions for LC-MS/MS analysis of DNA adducts was simplified and improved to increase throughput while maintaining high adduct selectivity. It was demonstrated that the StageTip composed of two sheets of a poly(styrene-divinylbenzene) copolymer disk could be easily prepared and proved useful for selective extraction of trace amounts of DNA adducts from a sample solution containing a great quantity of normal deoxynucleosides.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2116/analsci.30.519 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Platinum micromotors for mucosal penetration and colorectal cancer therapy.
Int J Pharm
January 2025
Clinical Center for Tumor Therapy, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. Electronic address:
The therapeutic outcomes of medications were restricted by the colonic mucosal barrier during the treatment of colorectal cancer (CRC). Micro/nanomotors can overcome the mucus barriers to reach deep colorectal tumors. In this study, we constructed a novel microsized PLGA-Pt micromotor (MM) driven by hydrogen peroxide (HO) to enhance drug delivery to the CRC tissues and achieve effective antitumor therapy.
View Article and Find Full Text PDFWintering loons in South Korea face an ongoing threat from polycyclic aromatic hydrocarbons: Shifting sources and potential DNA damage.
Environ Pollut
January 2025
Department of Biological Sciences, College of Natural Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea. Electronic address:
Diving birds, particularly those sharing coastal habitats with fishing grounds, are at risk from oil pollution. Despite documented cases of bird mortality, the specific role of oil pollution in these death remains unclear. To address this knowledge gap, this study examined polycyclic aromatic hydrocarbon (PAH) contamination, its sources, and its impact on loon health.
View Article and Find Full Text PDFInvestigating the origins of the mutational signatures in cancer.
Nucleic Acids Res
January 2025
Oxidative Stress Group, Department of Molecular Biosciences, University of South Florida, 4202 E. Fowler Avenue, Tampa, FL 33620, USA.
Most of the risk factors associated with chronic and complex diseases, such as cancer, stem from exogenous and endogenous exposures experienced throughout an individual's life, collectively known as the exposome. These exposures can modify DNA, which can subsequently lead to the somatic mutations found in all normal and tumor tissues. Understanding the precise origins of specific somatic mutations has been challenging due to multitude of DNA adducts (i.
View Article and Find Full Text PDFDesign and in vitro anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease.
Nucleic Acids Res
January 2025
School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Copper compounds with artificial metallo-nuclease (AMN) activity are mechanistically unique compared to established metallodrugs. Here, we describe the development of a new dinuclear copper AMN, Cu2-BPL-C6 (BPL-C6 = bis-1,10-phenanthroline-carbon-6), prepared using click chemistry that demonstrates site-specific DNA recognition with low micromolar cleavage activity. The BPL-C6 ligand was designed to force two redox-active copper centres-central for enhancing AMN activity-to bind DNA, via two phenanthroline ligands separated by an aliphatic linker.
View Article and Find Full Text PDFBracken Fern Carcinogen, Ptaquiloside, Forms a Guanine -Adduct in DNA.
J Agric Food Chem
January 2025
Centre for Chemical Biology, Department of Chemistry, Institute for Nucleic Acids, University of Sheffield, Brook Hill, Sheffield S3 7HF, U.K.
Bracken fern ( sp.) is a viable and vigorous plant with invasive potential, ingestion of which causes chronic illness and cancers in farm animals. Bracken is a suspected human carcinogen, and exposure can result from ingestion of bracken-contaminated water, dairy products, or meat derived from livestock grazing on bracken fern.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!