Genotyping and phylogenetic analysis of bovine viral diarrhea virus (BVDV) isolates in Kosovo.

Vet Ital

Kosovo Food and Veterinary Laboratory, Kosovo Food and Veterinary Agency, Zona industriale, 10 000 Pristina, Kosovo.

Published: December 2014

AI Article Synopsis

  • Three serum samples tested positive for BVDV using an Antigen ELISA method to study the virus's genetic diversity in Kosovo.
  • The samples, taken from heifers in 2011, underwent amplification through reverse transcription-polymerase chain reaction (RT-PCR), followed by sequencing and computer-assisted phylogenetic analysis.
  • All three isolates were confirmed to belong to the BVDV 1 genotype and 1b sub genotype, providing new insights into BVDV and marking the first documented cases of BVDV isolates in Kosovo.

Article Abstract

Three serum samples positive in Antigen ELISA BVDV have been tested to characterise genetic diversity of bovine viral diarrhea virus (BVDV) in Kosovo. Samples were obtained in 2011 from heifers and were amplified by reverse transcription-polymerase chain reaction, sequenced and analysed by computer-assisted phylogenetic analysis. Amplified products and nucleotide sequence showed that all 3 isolates belonged to BVDV 1 genotype and 1b sub genotype. These results enrich the extant knowledge of BVDV and represent the first documented data about Kosovo BVDV isolates.

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http://dx.doi.org/10.12834/VetIt.1304.11DOI Listing

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