Metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. There are controversy aspects about its teratogenicity. A teratogenic agent can alter morphology or subsequent function of the fetus. The aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by Metronidazole using FTIR micro spectroscopy. The mice were injected with metronidazole (60 mg/Kg) on gestation day 9. Fetuses were dissected on day 15 of gestation and morphological and histological studies on the fetus were carried out. Serial sectioning (10 μm) of normal and metronidazole-treated brains and livers were used for FTIR measurement in the wave number region of 600- 3600 cm(-1).The results showed that there were some variations between the fetus of normal and treated brain and liver. The band intensities in fetus brain and liver of test animals were reduced and shifted at 707 cm(-1), 1155 cm(-1), 1054 cm(-1), 1256 cm(-1) and 1219 cm(-1), 1453 cm(-1) and 1525 cm(-1), 1622 cm(-1), 1645 cm(-1) and 2944 cm(-1),while the band intensities were increased and shifted at 879 cm(-) (1) , 810 cm(-1), 1223 cm(-1), 1256 cm(-1) 1360 cm(-1), 1723 cm(-1). It was concluded that most of variations in brain and liver of Metronidazole treated fetuses are in amid bands, nucleic acid and carbohydrate related bands. Based on these findings FTIR spectroscopy can be a useful tool for bio diagnostic.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977059 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Computational and biochemical characterization of the immobilized esterase of Salinicoccus roseus for pesticide degradation.
Sci Rep
December 2024
Department of Botany, The University of Burdwan, Purba Bardhaman, 713104, West Bengal, India.
The continuous exposure of chemical pesticides in agriculture, their contamination in soil and water pose serious threat to the environment. Current study used an approach to evaluate various pesticides like Hexaconazole, Mancozeb, Pretilachlor, Organophosphate and λ-cyhalothrin degradation capability of esterase. The enzyme was isolated from Salinicoccus roseus.
View Article and Find Full Text PDFPhotonic platform coupled with machine learning algorithms to detect pyrolysis products of crack cocaine in saliva: A proof-of-concept animal study.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Innovation Center in Salivary Diagnostics and Nanobiotechnology, Department of Physiology, Institute of Biomedical Sciences, Federal University of Uberlandia (UFU), Uberlandia, MG, Brazil. Electronic address:
The non-invasive detection of crack/cocaine and other bioactive compounds from its pyrolysis in saliva can provide an alternative for drug analysis in forensic toxicology. Therefore, a highly sensitive, fast, reagent-free, and sustainable approach with a non-invasive specimen is relevant in public health. In this animal model study, we evaluated the effects of exposure to smoke crack cocaine on salivary flow, salivary gland weight, and salivary composition using Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy.
View Article and Find Full Text PDFExtraordinary SERS on nanostructured self-assembled silver films due to preliminary random nano-profiling of substrate and ultrasonic exposure of analyte during the deposition.
Spectrochim Acta A Mol Biomol Spectrosc
December 2024
Institute for Theoretical and Applied Electromagnetics RAS, Moscow 125412, Russia; Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia.
In this study, we have investigated the surface-enhanced Raman scattering (SERS) spectra of myoglobin on silver substrates with different morphology. The aim was to determine the optimal parameters of analyte and substrate preparation for obtaining of high-amplitude SERS spectra of proteins. It is shown that not only the morphology of the silver film, but also the method of analyte molecules deposition on the SERS substrate plays an important role.
View Article and Find Full Text PDFSpectroscopy and Dynamics of the Dipole-Bound States of -, -, and -Methylphenolate Anions.
J Phys Chem A
December 2024
Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.
A photodetachment and photoelectron spectroscopic study by employing a cryogenically cooled ion trap combined with a velocity-map imaging setup has been carried out to unravel the vibrational structures and autodetachment dynamics of the dipole-bound states (DBSs) of -, -, and -methylphenolate anions (-, -, and -CHPhO). The electron binding energy of the DBS increases monotonically with the increase of the neutral dipole moment to give respective values of 66 ± 15, 123 ± 18, or 154 ± 14 cm for the -, -, or -isomer. The different electron-donating effects of the methyl moieties in the three geometrically different isomers seem to be reflected in the experiment.
View Article and Find Full Text PDFOptimization of Collagen Extraction from Fish Scales Using Tris-Glycine Buffer: A Taguchi Methodological Approach.
Mar Drugs
December 2024
Discipline of Dietetics and Nutrition, College of Agriculture, University of KwaZulu-Natal, Durban 3201, South Africa.
Collagen, a critical biomaterial with wide applications in pharmaceuticals, cosmetics, and medical industries, can be sourced sustainably from fish scales. This study optimizes the extraction of collagen using Tris-Glycine buffer from fish scales via the Taguchi method. Various extraction parameters-buffer concentration, temperature, pH, and time-were evaluated to identify optimal conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!