AI Article Synopsis
- Transcriptional slippage is an error where RNA polymerase misaligns while copying DNA, impacting gene regulation and disease development.
- The bacteriophage λ N protein helps reduce this slippage within cells and in lab conditions by stabilizing the RNA/DNA hybrid.
- This study reveals the first known protein that affects transcriptional slippage and sheds light on how transcription termination and N-mediated antitermination work.
Article Abstract
Transcriptional slippage is a class of error in which ribonucleic acid (RNA) polymerase incorporates nucleotides out of register, with respect to the deoxyribonucleic acid (DNA) template. This phenomenon is involved in gene regulation mechanisms and in the development of diverse diseases. The bacteriophage λ N protein reduces transcriptional slippage within actively growing cells and in vitro. N appears to stabilize the RNA/DNA hybrid, particularly at the 5' end, preventing loss of register between transcript and template. This report provides the first evidence of a protein that directly influences transcriptional slippage, and provides a clue about the molecular mechanism of transcription termination and N-mediated antitermination.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4027172 | PMC |
| http://dx.doi.org/10.1093/nar/gku203 | DOI Listing |
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