AI Article Synopsis
- Cancer cells release tiny vesicles called extracellular vesicles (EVs) into their surroundings, which have potential as biomarkers, but detecting them in clinical samples is tough.
- The study introduces a quick and sensitive method to analyze EVs directly from blood samples of colorectal cancer patients, using antibodies and photosensitizer-beads for detection without needing to purify the EVs first.
- It highlights the use of the antigen CD147 found in cancer-related EVs for identifying colorectal cancer, marking a step forward in liquid biopsy techniques for better diagnosis and treatment.
Article Abstract
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3988821 | PMC |
| http://dx.doi.org/10.1038/ncomms4591 | DOI Listing |
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