AI Article Synopsis
Article Abstract
Under physiological and pathological conditions, extracellular vesicles (EVs) are present in the extracellular compartment simultaneously with soluble mediators. We hypothesized that cytokine effects may be modulated by EVs, the recently recognized conveyors of intercellular messages. In order to test this hypothesis, human monocyte cells were incubated with CCRF acute lymphoblastic leukemia cell line-derived EVs with or without the addition of recombinant human TNF, and global gene expression changes were analyzed. EVs alone regulated the expression of numerous genes related to inflammation and signaling. In combination, the effects of EVs and TNF were additive, antagonistic, or independent. The differential effects of EVs and TNF or their simultaneous presence were also validated by Taqman assays and ELISA, and by testing different populations of purified EVs. In the case of the paramount chemokine IL-8, we were able to demonstrate a synergistic upregulation by purified EVs and TNF. Our data suggest that neglecting the modulating role of EVs on the effects of soluble mediators may skew experimental results. On the other hand, considering the combined effects of cytokines and EVs may prove therapeutically useful by targeting both compartments at the same time.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11113439 | PMC |
| http://dx.doi.org/10.1007/s00018-014-1618-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The isolation of VCAM-1 endothelial cell-derived extracellular vesicles using microfluidics.
Extracell Vesicles Circ Nucl Acids
February 2024
Center for Engineering in Medicine & Surgery, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown - Boston, MA 02129, USA.
Vascular cell adhesion molecule-1 (VCAM-1) endothelial cell-derived extracellular vesicles (EC-EVs) are augmented in cardiovascular disease, where they can signal the deployment of immune cells from the splenic reserve. Endothelial cells in culture activated with pro-inflammatory tumor necrosis factor-α (TNF-a) also release VCAM-1 EC-EVs. However, isolating VCAM-1 EC-EVs from conditioned cell culture media for subsequent in-depth analysis remains challenging.
View Article and Find Full Text PDFAdipose mesenchymal stem cell-derived extracellular vesicles regulate PINK1/parkin-mediated mitophagy to repair high glucose-induced dermal fibroblast senescence and promote wound healing in rats with diabetic foot ulcer.
Acta Diabetol
December 2024
Department of Bone Surgery, The Second Affiliated Hospital, Guangzhou Medical University, No. 250 Changgang East Road, Haizhu District, Guangzhou, 510145, Guangdong Province, China.
Aims: Diabetic foot ulcers (DFUs) cause prominent morbidity and mortality. Adipose mesenchymal stem cell (ASC)-derived extracellular vesicles (EVs) show property in facilitating diabetic wound healing, and we explored their role in DFU rats.
Methods: ASCs were cultured in vitro, passaged and then identified by flow cytometry and induction of osteogenic/adipogenic differentiation.
Intestinal stem cell-derived extracellular vesicles ameliorate necrotizing enterocolitis injury.
Mol Cell Probes
December 2024
Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China; Key Laboratory of Birth Defects, Children's Hospital, Institutes of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address:
The therapeutic potential of intestinal stem cell-derived extracellular vesicles (ISCs-EVs) in necrotizing enterocolitis (NEC) remains largely unexplored. This research aims to investigate the therapeutic effects of ISCs-EVs on NEC. Lgr5-positive ISCs were screened from the small intestine of mice by flow cytometry, and ISCs-EVs were isolated by density gradient centrifugation.
View Article and Find Full Text PDFExtracellular vesicles released by ALL patients contain HNE-adducted proteins: Implications of collateral damage.
Free Radic Biol Med
December 2024
Department of Toxicology and Cancer Biology, University of Kentucky, USA; Markey Cancer Center, University of Kentucky, USA. Electronic address:
Off-target neuronal injury is a serious side-effect observed in cancer survivors. It has previously been shown that pediatric acute lymphoblastic leukemia (ALL) survivors have a decline in neurocognition compared to healthy age-matched counterparts. Elevated oxidative stress has been documented to be a mediator in off-target tissue damage in cancer survivors.
View Article and Find Full Text PDFA novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma.
Acta Obstet Gynecol Scand
November 2024
Prof. Ephraim Katzir Department of Biotechnology Engineering, Braude College of Engineering, St, Karmiel, Israel.
Introduction: Placental-derived extracellular vesicles (EVs) are nano-organelles that facilitate intercellular communication between the feto-placental unit and the mother. We evaluated a novel Multiple Microarray analyzer for identifying surface markers on plasma EVs that predict preterm delivery and preeclampsia compared to term delivery controls.
Material And Methods: In this prospective exploratory cohort study pregnant women between 24 and 40 gestational weeks with preterm delivery (n = 16), preeclampsia (n = 19), and matched term delivery controls (n = 15) were recruited from Bnai Zion Medical Center, Haifa, Israel.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!