We report a simple solution to the problem of quantitative densitometry of stained nitrocellulose paper. By immersing the paper in a household lubricating oil of matching refractive index, the light-scattering properties of the paper are largely eliminated, allowing precise transmission densitometry in any flat bed densitometer. The method was evaluated on immunochemically stained Western blots of the proteinases cathepsins B and L. An approximately linear relationship was found between the integrated absorbance of the stained zone and the logarithm of the amount of protease applied to the polyacrylamide gel over the range of 150 to 350 ng of cathepsin B and 50 to 250 ng of cathepsin L.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0003-2697(88)90364-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Associations of linear growth with body composition of perinatally HIV-infected African adolescents.
Br J Nutr
December 2024
Institute of Child Health, University College London, London, UK.
The prevalence of poor linear growth among African children with perinatally acquired HIV remains high. There is concern that poor linear growth may to lead to later total and central fat deposition and associated non-communicable disease risks. We investigated associations between height-for-age score (HAZ) and total and regional fat and lean mass measured by dual-energy X-ray absorptiometry, expressed as internal population scores, among 839 Zimbabwean and Zambian perinatally HIV-infected male and female adolescents aged 11-19 years.
View Article and Find Full Text PDFDevelopment of analytical equations for void fraction in biphasic systems using gamma radiation and MCNP6 code.
Appl Radiat Isot
December 2024
Instituto de Engenharia Nuclear - (IEN), Rua Hélio de Almeida 75, 21941-906, Cidade Universitária, RJ, Brazil. Electronic address:
This study presents the development of mathematical equations for calculating void fractions in pipes using gamma densitometry. A traditional measurement setup, consisting of aCs point source and a NaI(Tl) scintillator detector, was simulated using the Monte Carlo method via the MCNP6 code. To validate the proposed equations, water-gas biphasic models were simulated in tubes with square and cylindrical cross-sections, varying diameters, and radiation sources (Am, Cs, Co) through gamma-ray transmission.
View Article and Find Full Text PDFA high throughput immuno-affinity mass spectrometry method for detection and quantitation of SARS-CoV-2 nucleoprotein in human saliva and its comparison with RT-PCR, RT-LAMP, and lateral flow rapid antigen test.
Clin Chem Lab Med
May 2024
Department of Genetics and Genome Biology, Leicester Cancer Research Centre, University of Leicester, Leicester, UK.
Objectives: Many reverse transcription polymerase chain reaction (RT-PCR) methods exist that can detect SARS-CoV-2 RNA in different matrices. RT-PCR is highly sensitive, although viral RNA may be detected long after active infection has taken place. SARS-CoV-2 proteins have shorter detection windows hence their detection might be more meaningful.
View Article and Find Full Text PDFA Visible Light-Induced and ROS-Dependent Method for the Rapid Formation of a MOF Composite Membrane with Antibacterial Properties.
Int J Mol Sci
January 2023
College of Chemistry and Chemical Engineering, Donghua University, Shanghai 201620, China.
The diverse application potential of metal-organic framework (MOF) materials are currently limited by their challenging and complicated preparation processes. In this study, we successfully developed a novel strategy for the rapid synthesis of a sustainable MOF composite membrane under neutral conditions with improved physicochemical and antibacterial properties. Our reaction pipeline comprised visible light that induced the production of reactive oxygen species (ROS) from ZIF-8 particles, which facilitated the rapid oxidative polymerization of dopamine to polydopamine.
View Article and Find Full Text PDFBi-Directional Axial Transmission measurements applied in a clinical environment.
PLoS One
January 2023
Berlin-Brandenburg School for Regenerative Therapies, Charité - Universitätsmedizin Berlin, Germany.
Accurate measurement of cortical bone parameters may improve fracture risk assessment and help clinicians on the best treatment strategy. Patients at risk of fracture are currently detected using the current X-Ray gold standard DXA (Dual XRay Absorptiometry). Different alternatives, such as 3D X-Rays, Magnetic Resonance Imaging or Quantitative Ultrasound (QUS) devices, have been proposed, the latter having advantages of being portable and sensitive to mechanical and geometrical properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!