AI Article Synopsis
- The study reveals that urease-negative Campylobacter lari JCM2530(T) has two genes for major outer membrane proteins (MOMPs) and shows variability across different strains.
- Using PCR cloning and sequencing, researchers identified specific gene structures and transcriptional elements essential for protein production in five C. lari isolates.
- The analysis also uncovered an extra gene associated with the porA1 locus in a urease-positive C. lari strain, highlighting genetic diversity within this bacterial species.
Article Abstract
Southern hybridisation shows that urease-negative (UN) Campylobacter lari JCM2530(T) carries two putative major outer membrane protein (MOMP) genes. Sequences of approximately 2.1 kbp, encoding non-coding (NC) regions, with possible open reading frames (ORFs) for MOMP (porA1 or porA2) of approximately 1.2 kbp, NC regions and partial and putative Cla_0435 or Cla_1109 ORFs were identified in all five UN C. lari isolates examined, following polymerase chain reaction (PCR) cloning and sequencing. Each putative MOMP structural gene carried start and stop codons and ribosome binding sites of 1236-1278 bp in length. The putative sigma70 transcriptional promoter and the hypothetical rho-independent transcription terminator structures were also seen. Using Northern hybridisation, there was in vivo monocistronic MOMP gene transcription. In addition, in a Japanese urease-positive thermophilic Campylobacter (UPTC) CF89-12 strain, the porA1 gene locus, including an extra gene (approximately 2000 bp in length) was identified. The extra gene may occur within the porA1 gene locus in the eight UPTC isolates of the 23 C. lari isolates examined. Thus, a genetic heterogeneity occurred within the porA1 gene locus from some of the C. lari organisms including the UPTC CF89-12.
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| http://dx.doi.org/10.1080/09674845.2014.11669958 | DOI Listing |
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View Article and Find Full Text PDFArticle Synopsis
- The study reveals that urease-negative Campylobacter lari JCM2530(T) has two genes for major outer membrane proteins (MOMPs) and shows variability across different strains.
- Using PCR cloning and sequencing, researchers identified specific gene structures and transcriptional elements essential for protein production in five C. lari isolates.
- The analysis also uncovered an extra gene associated with the porA1 locus in a urease-positive C. lari strain, highlighting genetic diversity within this bacterial species.
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