AI Article Synopsis
- Photobleaching limits the effectiveness of fluorescence measurements by causing molecules to lose their ability to fluoresce or change their emission properties over time.
- Reducing photobleaching is crucial for enhancing fluorescence imaging, single molecule tracking, and the performance of biosensors.
- In this study, researchers utilized DNA self-assembly to create hybrid structures with fluorophores and gold nanoparticles, which increased photon emission before photobleaching by adjusting the nanoparticles' size.
Article Abstract
The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles' size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching.
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| http://dx.doi.org/10.1021/nl500841n | DOI Listing |
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