Skin and subcutaneous tissues are the origin of most common neoplasms affecting dogs, accounting for approximately one third of all tumors encountered in the species. Surgical excision is frequently the best chance for a cure; determining factors influencing the success of excision are vital for surgical management of cases. This work examined the shrinkage of skin of various lengths from three sites in formalin for both dogs and cats. Tissues were measured on the animal (initial measurement), at the time of excision (post-removal), and after formalin fixation (post-fixation). While shrinkage after tissue removal was found in samples from the thorax, abdomen, and rear leg in dogs and from the rear leg in cats, no significant shrinkage due to formalin fixation was detected in any sample except for the thoracic samples from the dog. Therefore, when determining where to make incisions to effect a surgical cure, initial measurements should take into account tissue shrinkage effects.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961154 | PMC |
| http://dx.doi.org/10.7717/peerj.307 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Examining the Formalin Fixation Method for Maintaining High RNA Quality in Surgical Lung Specimens.
Thorac Cancer
January 2025
Department of Thoracic and Cardiovascular Surgery, Faculty of Medicine, Saga University, Saga, Japan.
Background: Multiplex genetic testing is recommended when treating nonsmall cell lung cancer. A certain percentage of test failures in RNA assays owing to poor surgical specimen quality have been documented, and fixation failure is a possible cause. At our institution, sheet-like fixation is performed to reduce fixation time.
View Article and Find Full Text PDFThe Impact of Short-Term Formalin Fixation on Weight and Ventricular Dimensions in the Hearts of Cats and Small-to-Medium-Sized Dogs.
Vet Sci
January 2025
Department of Pathology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland.
Organ weight and size are important data collected during post-mortem examination not only in neoplastic diseases but also in other conditions, like cardiomyopathies. As post-mortem cardiac examination is challenging, it should be performed by experienced specialists. Nonetheless, the low number of referral centres in veterinary medicine requires the shipment of formalin-fixed specimens to perform detailed post-mortem cardiac examinations.
View Article and Find Full Text PDFTechnical Note: Application of Luminol and Leucomalachite Green Tests to Wischnewsky Spots After Formalin Fixation.
Am J Forensic Med Pathol
January 2025
From the Section of Legal Medicine, Department of Social Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Wischnewsky spots are disseminated, dark lesions in gastric mucosa reflecting hemorrhage associated with fatal hypothermia, and are a phenomenon well known to forensic pathologists. We applied luminol and leucomalachite green tests to formalin-fixed gastric mucosa with Wischnewsky spots in autopsy cases of hypothermia. Both luminol and leucomalachite green tests showed positive reactions.
View Article and Find Full Text PDFMultidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry.
Methods Cell Biol
January 2025
Pathology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address:
In recent years, significant advancements have been achieved in the development of multiplex imaging methodologies for immunophenotyping, enabling a comprehensive characterization of the complexity of tumor microenvironments. Imaging mass cytometry combines the detection of over 40 cellular targets with spatial information, enabling the identification of not only which cells are present in a tissue but also their localization relative to each other. Here, we present an easy-to-implement imaging mass cytometry workflow that ranges from antibody selection and testing to running a full panel.
View Article and Find Full Text PDFSingle-molecule localisation microscopy (SMLM) is feasible in human and animal formalin fixed paraffin embedded (FFPE) tissues in medical renal disease.
J Clin Pathol
January 2025
Pathology & Data Analytics, Leeds Institute of Medical Research at St. James's, School of Medicine, University of Leeds, Leeds, LS9 7TF, UK.
Aims: Establishment of a protocol for routine single-molecule localisation microscopy (SMLM) imaging on formalin fixed paraffin embedded (FFPE) tissue using medical renal disease including minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS).
Methods: Protocol for normal and diseased renal FFPE tissue was developed to investigate the clinical diagnostic potential of SMLM. Antibody concentrations were determined for confocal microscopy and transferred to SMLM.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!