Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091302 | PMC |
| http://dx.doi.org/10.1039/c4cc00053f | DOI Listing |
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