AI Article Synopsis
- The study investigated the presence of Wolbachia endosymbionts in the human parasite Dracunculus medinensis and related species, using various screening methods.
- All tested specimens, including D. medinensis, D. lutrae, and D. insignis, were found to be negative for Wolbachia, as confirmed by multiple molecular techniques and staining methods.
- These findings indicate that members of the Dracunculoidea superfamily likely do not harbor Wolbachia, suggesting that these endosymbionts are primarily confined to the Filarioidea group within the Spirurida order.
Article Abstract
Background: Wolbachia endosymbionts are a proven target for control of human disease caused by filarial nematodes. However, little is known about the occurrence of Wolbachia in taxa closely related to the superfamily Filarioidea. Our study addressed the status of Wolbachia presence in members of the superfamily Dracunculoidea by screening the human parasite Dracunculus medinensis and related species from wildlife for Wolbachia.
Findings: D. medinensis, D. lutrae and D. insignis specimens were all negative for Wolbachia colonization by PCR screening for the Wolbachia ftsZ, 16S rRNA and Wolbachia surface protein (wsp) sequences. The quality and purity of the DNA preparations was confirmed by amplification of nematode 18S rRNA and cytochrome c oxidase subunit I sequences. Furthermore, Wolbachia endobacteria were not detected by whole mount fluorescence staining, or by immunohistochemistry using a Wolbachia-specific antiserum. In contrast, positive control Brugia malayi worms were shown to harbour Wolbachia by PCR, fluorescence staining and immunohistochemistry.
Conclusions: Three examined species of Dracunculus showed no evidence of Wolbachia endobacteria. This supports that members of the superfamily Dracunculoidea are free of Wolbachia. Within the order Spirurida, these endosymbionts appear restricted to the Filarioidea.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994231 | PMC |
| http://dx.doi.org/10.1186/1756-3305-7-140 | DOI Listing |
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