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| http://dx.doi.org/10.1089/scd.2014.1500 | DOI Listing |
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Populations of very small embryonic-like stem cells (VSELs) (CD34+lin-CD45- and CD133+lin-CD45-), circulating in the peripheral blood of adults in small numbers, have been identified in several human tissues and together with the populations of hematopoietic stem cells (HSCs) (CD34+lin-CD45+) and CD133+lin-CD45+constitute a pool of cells with self-renewal and pluripotent stem cell characteristics. Using advanced cell staining and sorting strategies, we isolated populations of VSELs and HSCs for bulk RNA-Seq analysis to compare the transcriptomic profiles of both cell populations. Libraries were prepared from an extremely small number of cells; however, their good quality was preserved, and they met the criteria for sequencing.
View Article and Find Full Text PDFscRNA-seq revealed transcriptional signatures of human umbilical cord primitive stem cells and their germ lineage origin regulated by imprinted genes.
Sci Rep
November 2024
Laboratory of Regenerative Medicine, Center for Preclinical Studies and Technology, Medical University of Warsaw, Ul. Banacha 1B, Warsaw, Poland.
Article Synopsis
- Researchers identified a specific population of Very Small Embryonic-Like Stem Cells (VSELs) in human postnatal tissues such as bone marrow, peripheral blood, and umbilical cord blood that can differentiate into various tissue stem cells.
- Molecular analysis showed that these VSELs share similar characteristics with embryonic stem cells, including certain gene expressions and patterns of genetic imprinting.
- Using single-cell RNA sequencing, the study found that these human VSELs also exhibit subpopulations related to germline development and are influenced by factors that control differentiation and immune response.
Selected stem cell populations in pediatric acute lymphoblastic leukemia.
Front Immunol
October 2024
Department of Regenerative Medicine and Immune Regulation, Medical University of Bialystok, Bialystok, Poland.
Introduction: Acute lymphoblastic leukemia is characterized by a disturbed maturation of hematopoietic stem cells (HSCs) resulting in development of a malignant clone. Despite relatively positive outcome, there are still instances of disease relapse occurring due to ineffective disease eradication or primary leukemic clone alterations. Unclear significance of stem cells in the course of ALL led us to investigate and establish crucial changes in two stem cell populations - very small embryonic-like stem cells (VSELs) and HSCs during the induction phase of treatment.
View Article and Find Full Text PDFThe Phoenix of stem cells: pluripotent cells in adult tissues and peripheral blood.
Front Bioeng Biotechnol
July 2024
Dipartimento di Medicina Interna e Specialità Mediche (DIMI), Università Degli Studi di Genova, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
Pluripotent stem cells are defined as cells that can generate cells of lineages from all three germ layers, ectoderm, mesoderm, and endoderm. On the contrary, unipotent and multipotent stem cells develop into one or more cell types respectively, but their differentiation is limited to the cells present in the tissue of origin or, at most, from the same germ layer. Multipotent and unipotent stem cells have been isolated from a variety of adult tissues, Instead, the presence in adult tissues of pluripotent stem cells is a very debated issue.
View Article and Find Full Text PDFBlastocyst-like Structures in the Peripheral Retina of Young Adult Beagles.
Int J Mol Sci
May 2024
Department of Ophthalmology, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Osaka, Japan.
In this immunohistological study on the peripheral retina of 3-year-old beagle dogs, excised retina specimens were immunostained with antibodies against nestin, Oct4, Nanog, Sox2, CDX2, cytokeratin 18 (CK 18), RPE65, and YAP1, as well as hematoxylin and DAPI, two nuclear stains. Our findings revealed solitary cysts of various sizes in the inner retina. Intriguingly, a mass of small round cells with scant cytoplasms was observed in the cavity of small cysts, while many disorganized cells partially occupied the cavity of the large cysts.
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