The authors have defined the optimal conditions for the determination of granulocyte luminol-dependent chemiluminescence (LDCL) in normal donors; the granulocytes have been isolated from small volumes of blood. Granulocytic LDCL has been studied in granulocyte exposure to zymosan, zymosan-activated serum, phorbol-myristate acetate, and FMLP (formyl-L-methionyl-leucyl-phenylalanine) synthetic tripeptide. Significant individual differences in the granulocyte activities in normal subjects have been revealed.
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