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Thin-film luminescent sensors were developed to measure dissolved oxygen at very small volumes, specifically for tracking oxygen consumption in individual cells.
The study explored various platinum porphyrin sensor materials and used microscopic methods for measurements, while applying convolution theory to analyze unexpected results like red luminescent emissions.
A new technique was created for quickly determining exponential decay lifetime using long-pulsed luminescence, which significantly enhances signal quality if self-illumination is properly managed during experiments.*
Article Abstract
Thin-film luminescent sensors were used to measure dissolved oxygen in picoliter volumes for the purpose of monitoring single-cell oxygen consumption rates, and that work served as the motivation for the development of the method described here. A few different platinum porphyrin sensor materials were examined, with all measurements conducted microscopically. By employing convolution theory to understand observed responses, including an unexpected red luminescent emission from an optic, we developed a new, rapid method for the determination of exponential decay lifetime. This new method of long-pulsed luminescence offers substantially improved signal-to-noise ratios for detected signals as long as self-illumination sources are carefully controlled in the experimental set-up.
