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Investigation of deletion of 22pb in KIR2DS4 gene in a population of southern Brazil. | LitMetric
Background : The aim of this study was to investigate the distribution of full-length and deleted variants of KIR2DS4 in a population of southern Brazil and compare the results with other populations, as well as comparing two techniques, PCR-SSP and PCR-SSO, for typing of variants.Methods : 258 individuals from southern Brazil were analysed by PCR-SSO ("polymerase chain reaction-sequence specific oligonucleotides", One Lambda, Inc., Canoga Park, CA), of which 161 were also analysed by PCR-SSP.Results : The study population showed similarities with other Caucasian populations; 46.5% of individuals had only KIR2DS4 variants, 21.3% had the full-length form and 25.1% had both forms.Conclusion : The frequencies found in both groups (genotyped by PCR-SSP and PCR-SSO) were 100% concordant.
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