Recently, novel Klebsiella pneumoniae J2B, which grows rapidly on glycerol as the carbon source without forming pathogenic and sticky lipopolysaccharides, was isolated. Current study examined the ability of K. pneumoniae J2B to produce 1,3-propanediol (PDO) from glycerol. To this end, a deletion mutant for lactate formation was constructed. The ldhA mutant strain produced negligible lactate but more 2,3-butanediol (BDO). When K. pneumoniae ΔldhA was cultivated in glycerol fed-batch mode, the PDO titer of 58.0 g/L with a yield of 0.35 g/g and an overall volumetric productivity of 1.3g/L/h were obtained. BDO was the main byproduct (26.6g/L). Less than 10 g/L of the other metabolites was produced. As PDO and other metabolites accumulated, the rate of PDO production decreased significantly due mainly to the toxic effects of these metabolites. This study highlights the potential of newly isolated K. pneumoniae J2B for the production of PDO from glycerol.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biortech.2014.01.126 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of Klebsiella pneumoniae J2B as microbial cell factory for the production of 1,3-propanediol from glucose.
Metab Eng
November 2020
School of Chemical and Biomolecular Engineering, Pusan National University, Busan, South Korea; School of Energy and Chemical Engineering, UNIST, Ulsan, South Korea. Electronic address:
1,3-Propanediol (1,3-PDO) is an important platform chemical which has a wide application in food, cosmetics, pharmaceutical and textile industries. Its biological production using recombinant Escherichia coli with glucose as carbon source has been commercialized by DuPont, but E. coli cannot synthesize coenzyme B which is an essential and expensive cofactor of glycerol dehydratase, a core enzyme in 1,3-PDO biosynthesis.
View Article and Find Full Text PDFMetabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol: Reduction of acetate and other by-products.
Bioresour Technol
November 2017
School of Chemical and Biomolecular Engineering, Pusan National University, 2, Busandaehak-ro 63 beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea; School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Ulsan 44919, Republic of Korea. Electronic address:
Production of 3-hydroxypropionic acid (3-HP) or 1,3-propanediol (1,3-PDO) production from glycerol is challenging due to the problems associated with cofactor regeneration, coenzyme B synthesis, and the instability of pathway enzymes. To address these complications, simultaneous production of 3-HP and 1,3-PDO, instead of individual production of each compound, was attempted. With over-expression of an aldehyde dehydrogenase, recombinant Klebsiella pneumoniae could co-produce 3-HP and 1,3-PDO successfully.
View Article and Find Full Text PDFMetabolic engineering of Klebsiella pneumoniae J2B for the production of 1,3-propanediol from glucose.
Bioresour Technol
December 2017
School of Chemical and Biomolecular Engineering, Pusan National University, Busan, Republic of Korea; School of Energy and Chemical Engineering, UNIST, Ulsan, Republic of Korea. Electronic address:
The production of 1,3-propanediol (1,3-PDO) from glucose was investigated using Klebsiella pneumoniae J2B, which converts glycerol to 1,3-PDO and synthesize an essential coenzyme B. In order to connect the glycolytic pathway with the pathway of 1,3-PDO synthesis from glycerol, i.e.
View Article and Find Full Text PDFGlycerol-fed microbial fuel cell with a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonae J2B.
J Ind Microbiol Biotechnol
October 2016
School of Chemical and Biomolecular Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
Glycerol is an attractive feedstock for bioenergy and bioconversion processes but its use in microbial fuel cells (MFCs) for electrical energy recovery has not been investigated extensively. This study compared the glycerol uptake and electricity generation of a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonia J2B in a MFC with that of a single species inoculated counterpart. Glycerol was metabolized successfully in the co-culture MFC (MFC-J&M) with simultaneous electricity production but it was not utilized in the MR-1 only MFC (MFC-M).
View Article and Find Full Text PDFEffects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B.
Bioresour Technol
August 2016
School of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangeon-dong, Geumjeong-gu, Busan 609-735, Republic of Korea. Electronic address:
The current study investigates the impact of mutation of 2,3-butanediol (BDO) formation pathway on glycerol metabolism and 1,3-propanediol (PDO) production by lactate dehydrogenase deficient mutant of Klebsiella pneumoniae J2B. To this end, BDO pathway genes, budA, budB, budC and budO (whole-bud operon), were deleted from K. pneumoniae J2B ΔldhA and the mutants were studied for glycerol metabolism and alcohols (PDO, BDO) production.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!