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Glyphosate is a broad-spectrum herbicide that kills most vascular plant weeds but is ineffective against many bryophytes. Glyphosate competitively inhibits the enolpyruvyl transferase enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). EPSPS catalyzes the production of 5-enolpyruvylshikimate-3-phosphate (EPSP)-an intermediate in the shikimate pathway-from shikimate-3-phosphate (S3P) and phosphoenolpyruvate (PEP) substrates.

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Article Synopsis
  • Glyphosate use has led to the evolution of resistant weed populations, prompting a study focused on a glyphosate-resistant population (R-JX) from China.
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  • Pre-treatment with a P450 inhibitor reduced glyphosate resistance, while overexpressing the target gene in rice demonstrated that gene overexpression contributes to glyphosate resistance in these weed populations.
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