Cell-free protein synthesis: search for the happy middle.

Biotechnol J

Department of Bioengineering, University of California, Berkeley, CA, USA; Energy Biosciences Institute, University of California, Berkeley, CA, USA.

Published: May 2014

Cell-free protein synthesis (CFPS) is a versatile technique gaining popularity because it allows researchers access to on-demand production of proteins. This Commentary by Zachary Russ and John Dueber discusses the latest research article by Rui Gan and Michael Jewett, which reports a convenient and cost-effective methodology for the preparation of Saccharomyces cerevisiae-based CFPS reactions.

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http://dx.doi.org/10.1002/biot.201400071DOI Listing

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