AI Article Synopsis
- Molecular templates enhance the concentration of specific reactants, significantly speeding up chemical reactions, particularly those involving nucleic acids.
- The study introduces the first instance of using a protein template to facilitate the N-terminal linkage of two peptides.
- This protein-templated reaction shows over 1000 times faster reaction rates and high selectivity, successfully functioning even in complex biological environments like crude cell lysate.
Article Abstract
Molecular templates bind particular reactants, thereby increasing their effective concentrations and accelerating the corresponding reaction. This concept has been successfully applied to a number of chemical problems with a strong focus on nucleic acid templated reactions. We present the first protein-templated reaction that allows N-terminal linkage of two peptides. In the presence of a protein template, ligation reactions were accelerated by more than three orders of magnitude. The templated reaction is highly selective and proved its robustness in a protein-labeling reaction that was performed in crude cell lysate.
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| http://dx.doi.org/10.1002/anie.201400681 | DOI Listing |
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