Structure of the O-polysaccharide of Photorhabdus temperata subsp. temperata XlNach(T) containing a novel branched monosaccharide, 3,6-dideoxy-4-C-[(S)-1,2-dihydroxyethyl]-d-xylo-hexose. | LitMetric
O-Polysaccharide was isolated from the lipopolysaccharide of an entomopathogenic bacterium Photorhabdus temperata subsp. temperata XlNach(T). Sugar analysis after full acid hydrolysis of the polysaccharide revealed D-glucose, D-mannose, D-galactose, D-GalNAc, and a branched monosaccharide, 3,6-dideoxy-4-C-[(S)-1',2'-dihydroxyethyl]-D-xylo-hexose (Sug), which was isolated as a 1,2'-anhydro furanose derivative. The following structure of the polysaccharide was established by 1D and 2D 1H and 13C NMR spectroscopy:
The complete genome sequences of two bacteria isolated from an entomopathogenic nematode are reported. The genome sizes of sp. strain FFPRI_1 and strain FFPRI_2 are 7,521,420 bp long with 63% G+C content and 5,483,510 bp long with 43.
In Korea, there are two maggot species in the Delia genus that commonly infest the roots and stems of the Welsh onion, thus causing serious economic damage on the crop at the seedling stage. In this study, the seedcorn maggot (Delia platura) was detected in onion fields in two different localities in Korea. After overwintering, maggot infestations occurred throughout the entire growing seasons from transplantation to harvest, but their specific patterns of occurrence varied in the two localities examined.
and bacterial symbionts of entomopathogenic nematodes and , respectively, have several biological activities including insecticidal and antimicrobial activities. Thus, XnChi, XhChi, and PtChi, chitinases of , , and isolated from Korean indigenous EPNs GJ1-2, GJ11-1, and GJ1-2 were cloned and expressed in BL21 to compare their biological activities. Chitinase proteins of these bacterial symbionts purified using the Ni-NTA system showed different chitobiosidase and endochitinase activities, but N-acetylglucosamidinase activities were not shown in the measuring of chitinolytic activity through N-acetyl-D-glucosarmine oligomers.
An entomopathogenic bacterium, subsp. , is mutualistic to its host nematode, . The infective juvenile nematodes enter target insects through natural openings and release the symbiotic bacteria into the insect hemocoel.
For decades, transcription of -operon was considered being constitutive. Therefore, this -operon has been used for measurements in non-specific bacterial luminescent biosensors. Here, the expression of -operon under high temperature was studied.
