The amplification of telomerase component (TERC) gene could play an important role in generation and treatment of haematological malignancies. This present study was aimed to investigate copy number amplification status of TERC gene in chronic myeloid leukaemia (CML) patients who were being treated with imatinib mesylate (IM). Genomic DNA was extracted from peripheral blood of CML-IM Resistant (n=63), CML-IM Respond (n=63) and healthy individuals (n=30). TERC gene copy number predicted (CNP) and copy number calculated (CNC) were determined based on Taqman® Copy Number Assay. Fluorescence in situ hybridization (FISH) analysis was performed to confirm the normal signal pattern in C4 (calibrator) for TERC gene. Nine of CML patients showed TERC gene amplification (CNP=3), others had 2 CNP. A total of 17 CML patients expressed CNC>2.31 and the rest had 2.31>CNC>1.5. TERC gene CNP value in healthy individuals was 2 and their CNC value showed in range 1.59-2.31. The average CNC TERC gene copy number was 2.07, 1.99 and 1.94 in CML- IM Resistant patients, CML-IM Respond and healthy groups, respectively. No significant difference of TERC gene amplification observed between CML-IM Resistant and CML-IM Respond patients. Low levels of TERC gene amplification might not have a huge impact in haematological disorders especially in terms of resistance towards IM treatment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7314/apjcp.2014.15.4.1863 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hemophagocytic lymphohistiocytosis as the initial manifestation of bone marrow failure in a child with a TERC variant telomere biology disorder.
Ther Adv Rare Dis
January 2025
Department of Pediatrics, Hospital Nacional Edgardo Rebagliati Martins, Lima, Peru.
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening systemic hyperinflammatory syndrome, rarely associated with bone marrow failure (BMF). Telomere biology disorders (TBD) are caused by inherited defects in telomerase processes and can have heterogeneous presentations including idiopathic pulmonary fibrosis, cirrhosis, and BMF. We report a case of a 10-year-old male from Lima, Peru, who presented with HLH as the initial manifestation of a TBD.
View Article and Find Full Text PDFFirst report on comprehensive genomic analysis of a multidrug-resistant Enterobacter asburiae isolated from diabetic foot infection from Bangladesh.
Sci Rep
January 2025
Department of Microbiology, University of Dhaka, Dhaka, 1000, Bangladesh.
Enterobacter asburiae (E. asburiae) is a gram-negative rod-shaped bacterium which has emerging significance as an opportunistic pathogen having high virulence pattern and drug resistant properties. In this study, we present the detailed analysis of the whole genome sequence of a multidrug-resistant (MDR) E.
View Article and Find Full Text PDFIntegration of eQTL and multi-omics comprehensive analysis of triacylglycerol synthase 1 (TGS1) as a prognostic and immunotherapeutic biomarker across pan-cancer.
Int J Biol Macromol
January 2025
The Second Clinical Medical College, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, PR China; Children's Medical Center, The Second Hospital of Shandong University, Jinan 250033, PR China. Electronic address:
An increasing number of expression quantitative trait loci (eQTLs) have been linked to tumorigenesis. In this study, we used Mendelian randomization (MR) to identify a novel cancer susceptibility gene, Trimethylguanosine Synthase 1 (TGS1). TGS1-induced hypermethylation at the 5' end of human telomerase RNA (hTR) impedes hTR accumulation, decreasing telomerase assembly factor levels and thus limiting telomere elongation, a crucial factor in tumor progression.
View Article and Find Full Text PDFHot Spots of Site-Specific Integration into the Chromosome.
Int J Mol Sci
September 2024
Department of Biology, University of Florence, 50019 Sesto Fiorentino, Italy.
The diversity of phage-related sequences (PRSs) and their site-specific integration into the genomes of nonpathogenic, agriculturally valuable, nitrogen-fixing root nodule bacteria, such as , were evaluated in this study. A total of 314 PRSs, ranging in size from 3.24 kb to 88.
View Article and Find Full Text PDFPeripartum depression symptom trajectories, telomere length and genotype, and adverse childhood experiences.
BMC Psychiatry
October 2024
Department of Women's and Children's Health, Science for Life Laboratory, Uppsala University, Box 593, Uppsala, 751 24, Sweden.
Article Synopsis
- The study investigates the link between telomere length (TL) and peripartum depression (PPD), examining how adverse childhood experiences (ACE) and genetic variations affect this relationship.
- Results indicate that shorter TL is associated with more severe PPD symptoms, particularly at pregnancy week 32 and six weeks postpartum, highlighting a potential biological marker for understanding PPD.
- ACE plays a significant role in moderating the TL-PPD connection, with individuals experiencing greater ACE showing a more pronounced shortening of TL in persistent PPD cases compared to healthy controls.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!