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| http://dx.doi.org/10.3389/fmicb.2014.00081 | DOI Listing |
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Development of a qPCR assay to identify and differentiate insect-associated strains of the complex.
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Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Werribee, Victoria, Australia.
The complex contains important opportunistic pathogens of humans and vertebrate animals, as well as insects and other invertebrates. To date, the methods used for the identification of species within the genus , including PCR assays, have poor discriminatory power and may require further molecular typing or genomic sequence analysis to determine clinical relevance. We developed a duplex TaqMan probe-based quantitative real-time PCR (qPCR) assay targeting the gene, which is involved in chitin degradation and transport, and the gene, which is involved in urease production.
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Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210036, China.
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View Article and Find Full Text PDFActivity of Aztreonam-avibactam and other β-lactamase inhibitor combinations against Gram-negative bacteria isolated from patients hospitalized with pneumonia in United States medical centers (2020-2022).
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Element Iowa City (JMI Laboratories), 345 Beaver Kreek Centre, Suite A North Liberty, Iowa, IA, 52317, USA.
Background: Initial antimicrobial therapy for pneumonia is frequently empirical and resistance to antimicrobial agents represents a great challenge to the treatment of patients hospitalized with pneumonia. We evaluated the frequency and antimicrobial susceptibility of Gram-negative bacteria causing pneumonia in US hospitals.
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This work describes the synthesis, characterization, and antibacterial properties of four bile acid-triclosan conjugates. The in vitro antibacterial activity of synthetic bile acid-triclosan conjugates was investigated against a panel of Gram-positive and Gram-negative bacteria. Conjugates and show high activity against (ATCC25922), with IC values of 2.
View Article and Find Full Text PDFThe current investigation focuses on synthesizing Ag-Fe bimetallic nanoparticles (AgFe-BMNPs) using cell-free filtrates of the as a novel fungal reducing agent. The optical, morphological, and surface properties of these fungus-fabricated AgFe-BMNPs and their monometallic counterparts (AgNPs and FeNPs) were analyzed using sophisticated nanotechnology instruments. The UV-visible spectrum showed peaks at 231 nm and 415 nm for BMNPs and 450 nm and 386 nm for AgNPs and FeNPs, respectively.
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