AI Article Synopsis
- The past few years have seen significant advancements in novel trans-acting elements like TALEs, CRISPR/Cas, and sRNAs, which can target specific DNA or RNA sequences with ease.
- These tools can effectively suppress gene expression at various levels without the need to alter the target DNA directly.
- Researchers in synthetic biology, particularly those focused on eukaryotes, are now exploring these elements for genome regulation, editing, and epigenetic research, as well as their potential applications in prokaryotic systems.
Article Abstract
The last several years have witnessed an explosion in the understanding and use of novel, versatile trans-acting elements. TALEs, CRISPR/Cas, and sRNAs can be easily fashioned to bind any specific sequence of DNA (TALEs, CRISPR/Cas) or RNA (sRNAs) because of the simple rules governing their interactions with nucleic acids. This unique property enables these tools to repress the expression of genes at the transcriptional or post-transcriptional levels, respectively, without prior manipulation of cis-acting and/or chromosomal target DNA sequences. These tools are now being harnessed by synthetic biologists, particularly those in the eukaryotic community, for genome-wide regulation, editing, or epigenetic studies. Here we discuss the exciting opportunities for using TALEs, CRISPR/Cas, and sRNAs as synthetic trans-acting regulators in prokaryotes.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162867 | PMC |
| http://dx.doi.org/10.1016/j.copbio.2014.02.010 | DOI Listing |
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