AI Article Synopsis
- Gold nanoparticles conjugated with DNA are increasingly used for biomedical applications due to their unique surface chemistry, particularly those created via laser methods.
- The study investigates the attachment process of DNA to these laser-generated nanoparticles using Dynamic Light Scattering and UV-Vis spectroscopy, noting changes in hydrodynamic radius and spectral behavior.
- Dynamic Light Scattering proves to be an effective method for qualitatively assessing the binding of DNA to gold nanoparticles, making it a valuable tool for monitoring variations in the conjugation process.
Article Abstract
Gold nanoparticles (AuNPs) conjugated to DNA are widely used for biomedical targeting and sensing applications. DNA functionalization is easily reached on laser generated gold nanoparticles because of their unique surface chemistry, not reproducible by other methods. In this context, we present an extensive investigation concerning the attachment of DNA to the surface of laser generated nanoparticles using Dynamic Light Scattering and UV-Vis spectroscopy. The DNA conjugation is highlighted by the increase of the hydrodynamic radius and by the UV-Vis spectra behavior. Our investigation indicates that Dynamic Light Scattering is a suitable analytical tool to evidence, directly and qualitatively, the binding between a DNA molecule and a gold nanoparticle, therefore it is ideal to monitor changes in the conjugation process when experimental conditions are varied.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953011 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0089048 | PLOS |
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