Rectal/vaginal specimens (n = 97) were collected in parallel using ESwab and Liquid Stuart (LS) rayon fiber wrapped swab collection devices. Each collection device was used to directly inoculate culture medium and LIM broth. Medium inoculation by ESwab was conducted using the Walk-Away specimen processor (WASP). Medium inoculation by the LS device was conducted manually. The sensitivities of ESwab and LS upon direct plating were 93.8% and 87.5%, respectively, and increased to 96.9% and 90.6%, respectively, following broth enrichment.
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http://dx.doi.org/10.1128/JCM.00374-14 | DOI Listing |
Front Cell Infect Microbiol
January 2022
Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
Objective: Automation is increasingly being applied in clinical laboratories; however, preanalytical processing for microbiology tests and screening is still largely performed using manual methods owing to the complex procedures involved. To promote automation of clinical microbiology laboratories, it is important to assess the performance of automated systems for different specimen types separately. Therefore, the aim of this study was to explore the potential clinical application of the Copan Walk Away Specimen Processor (WASP) automated preanalytical microbiology processing system in the detection of pathogens in female reproductive tract specimens and its feasibility in optimizing diagnostic procedures.
View Article and Find Full Text PDFBMC Res Notes
August 2019
Epigenomics AG, Berlin, Germany.
Objective: DNA methylation analysis via real-time PCR or other analytical techniques requires purified bisulfite converted DNA. We report on an automated high throughput solution for DNA extraction, bisulfite-conversion, and purification of 96 samples with an input volume of up to 3.5 mL of plasma or urine, using reagents from the commercially available Epi BisKit.
View Article and Find Full Text PDFDiagn Microbiol Infect Dis
May 2019
Department of Microbiology, University Health Network/Sinai Health System, Toronto, Ontario, M5G 1X5, Canada.
The isolation of stool pathogens is difficult due to their fastidious nature and the rapid overgrowth of fecal flora. In this study, we evaluate the preservation and isolation of enteric pathogens from stool using the automated COPAN Walk-Away Specimen Processor (WASP®) in conjunction with FecalSwab™ and selenite media. Pathogen viability and fecal commensal abundance were stable in FecalSwab™ media under both room-temperature and refrigerated incubation conditions, resulting in a significantly increased number of well-isolated pathogen colonies observed when compared to samples incubated in modified Cary-Blair media.
View Article and Find Full Text PDFMethods Mol Biol
February 2018
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR, 97331, USA.
Surface plasmon resonance (SPR) is a physical process that allows label-free and real-time detection of biomolecular interactions. SPR provides a rapid and quantitative method for studying interactions of macromolecules such as proteins and nucleic acids. Antisense Morpholino oligomers are widely used to regulate gene expression and the US FDA has approved a Morpholino drug for treatment of Duchenne muscular dystrophy.
View Article and Find Full Text PDFLab Med
February 2016
Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy
Background: Although automation has become widely utilized in certain areas of diagnostic testing, its adoption in diagnostic microbiology has proceeded much more slowly.
Objective: To describe our real-world experience of integrating an automated instrument for diagnostic microbiology (Walk-Away Specimen Processor, WASPLab) within a model of total laboratory automation (TLA).
Methods: The implementation process was divided into 2 phases.
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