MicroRNAs (miRNAs) are small regulatory RNAs (size ∼21nt to ∼25nt) that can be used as biomarkers of disease diagnosis, and efforts have been directed towards the invention of a rapid, simple and sequence-selective detection method for miRNAs. We recently developed a DNA/silver nanoclusters (AgNCs)-based turn-off fluorescence method in the presence of target miRNA. To further advance our method toward multiplex miRNA detection in solution, the design of various fluorescent DNA/AgNCs probes was essential. Therefore, tethering of DNA-12nt scaffolds with 9 different AgNCs emitters to target-sensing DNA sequences was investigated. Interestingly, for the creation of spectrally different DNA/AgNCs probes, not only were the emitters encapsulated in 9 different DNA-12nt scaffolds necessary but the tethered target-sensing DNA sequences are also crucial to tune the fluorescence across the visible to infra-red region. In this study, we obtained three spectrally distinctive emitters of each DNA/AgNCs probes such as green, red, and near-infrared (NIR) fluorescence. Using these DNA/AgNCs probes, we here show a proof of concept for a rapid, one-step, in-solution multiplex miRNA detection method.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c3an02150e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dual-mode ratiometric optical sensor for rapid visual detection of Hg with poly(adenine)-assisted silver nanoclusters anchoring on tetrahedral DNA framework.
Mikrochim Acta
January 2025
College of Chemistry and Environmental Engineering, Sichuan University of Science and Engineering, Zigong, 643000, P.R. China.
Cytosine-rich and poly(adenine)-tailed tetrahedral DNA framework (TDF) is designed as template (A-TDF) for anchoring silver nanoclusters (AgNCs) and igniting the dual-color fluorescence of AgNCs. The resultant DNA-AgNCs simultaneously emits red and green fluorescence, and the quantum yield of red fluorescence is as high as 44.8%.
View Article and Find Full Text PDFLabel-free miRNA fluorescent biosensors based on duplex-specific nucleases and silver nanoclusters.
Analyst
January 2025
School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China.
MicroRNAs (miRNAs) are considered reliable biomarkers for a variety of diseases. However, their low abundance in organisms and high sequence similarity of homologous miRNAs make their accurate detection challenging. Here, we constructed a novel fluorescent biosensor for the detection of miRNA-155, a potential biomarker of neuroinflammation, based on duplex-specific nuclease (DSN) assisted amplification and DNA-templated silver nanoclusters (DNA-AgNCs) as fluorescence signal probes.
View Article and Find Full Text PDFA label-free activatable biosensor for detection of exosomal microRNAs based on DNA-AgNCs and hairpin type nucleic acid probes.
Anal Methods
February 2024
Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha, 410013, China.
Exosomal microRNA (miRNA) is a potential biomarker for cancer diagnosis, metastasis, and treatment. detection of exosomal miRNA is an attractive option due to its simplicity and high accuracy. However, exosomal miRNA detection has encountered challenges because of the low target abundance of targets and limited probe permeability.
View Article and Find Full Text PDFConstruction of an electrochemical-fluorescent dual-mode sensor with a dual-mode signal AgNC probe synthesized from cytosine-rich DNA for OTA detection.
J Mater Chem B
September 2023
College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 450001, PR China.
Herein, we have used DNA-silver nanocluster (DNA-AgNC) signal probes with both electrochemical and fluorescent signals for the first time to construct an electrochemical-fluorescent dual-mode sensor. The sensor has an easy-to-prepare dual-signal property combined with the magnetic separation technique for dual-mode detection of ochratoxin A (OTA). In the absence of OTA, the DNA strand used to synthesize AgNCs was not available in the system after magnetic separation.
View Article and Find Full Text PDFA DNA-Stabilized Ag Cluster with Excitation-Intensity-Dependent Dual Emission.
Angew Chem Int Ed Engl
September 2023
Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
DNA-stabilized silver nanoclusters (DNA-AgNCs) are easily tunable emitters with intriguing photophysical properties. Here, a DNA-AgNC with dual emission in the red and near-infrared (NIR) regions is presented. Mass spectrometry data showed that two DNA strands stabilize 18 silver atoms with a nanocluster charge of 12+.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!