Background: Factors associated with Mycobacterium tuberculosis (Mtb) strain success over time in high burdened communities are unknown.
Methods: Mtb isolates collected over 10 years from sputum-positive tuberculosis (TB) patients resident in the study site underwent IS6110-based restriction fragment length polymorphism analysis. Clinical, demographic and social data were extracted from clinic records and interviewer-administered questionnaires. Strains were defined as persistently successful, transiently successful or unsuccessful based on the average number of cases per year and their continued presence over time.
Results: Genotyping data were available on 789 TB cases. Of the 311 distinct Mtb strains (≥6 bands) identified, 247 were categorized as unsuccessful strains, 12 transiently successful and 10 persistently successful strains. Strain success was not associated with age, gender, antiretroviral use or social factors. Persistently successful strains were less likely to be drug-resistant compared with transiently successful strains [odds ratio (OR): 0.13; 95% confidence interval (CI): 0.04 - 0.5]. Persistently successful strains were positively associated with host HIV-infection compared with unsuccessful strains, but this finding was not robust in sensitivity analyses.
Conclusions: Pathogen characteristics appear to play a greater role in Mtb strain success compared with social or host factors. This study supports the need for further investigations into the role of pathogen characteristics in strain success.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121554 | PMC |
| http://dx.doi.org/10.1093/ije/dyu044 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Whole-genome automated assembly pipeline for strains from reference, and clinical samples using the integrated CtGAP pipeline.
NAR Genom Bioinform
March 2025
Departments of Medicine and Pediatrics, Division of Infectious Diseases and Global Health, University of California San Francisco School of Medicine, 550 16th Street, 4th Floor Mission Hall, San Francisco, CA, 94158, USA.
Whole genome sequencing (WGS) is pivotal for the molecular characterization of ()-the leading bacterial cause of sexually transmitted infections and infectious blindness worldwide. WGS can inform epidemiologic, public health and outbreak investigations of these human-restricted pathogens. However, challenges persist in generating high-quality genomes for downstream analyses given its obligate intracellular nature and difficulty with propagation.
View Article and Find Full Text PDFTriacylglycerol mobilization underpins mitochondrial stress recovery.
Nat Cell Biol
January 2025
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA.
Mitochondria are central to myriad biochemical processes, and thus even their moderate impairment could have drastic cellular consequences if not rectified. Here, to explore cellular strategies for surmounting mitochondrial stress, we conducted a series of chemical and genetic perturbations to Saccharomyces cerevisiae and analysed the cellular responses using deep multiomic mass spectrometry profiling. We discovered that mobilization of lipid droplet triacylglycerol stores was necessary for strains to mount a successful recovery response.
View Article and Find Full Text PDFCustomized Design of Host-Independent T7 Expression System (HITES) for a Broad Host Range.
J Biotechnol
January 2025
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:
Efficient methods and universal DNA elements are eagerly required for the expression of proteins and the production of target chemicals in synthetic biology and metabolic engineering. This paper develops a customized-design approach by utilizing the host-independent T7 expression system (HITES), which facilitates the rational design and rapid construction of T7 expression systems. Firstly, the EL (Upper-limit value of initial enzyme activity) value is discovered to play a pivotal factor in the successful construction of the T7 expression system, different host strains exhibit varying EL values, and this study presents a method to measure the EL values.
View Article and Find Full Text PDFLinear Plasmids in Micrococcus: Insights Into a Common Ancestor and Transfer by Conjugation.
Environ Microbiol
January 2025
Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Técnicas, San Miguel de Tucumán, Tucumán, Argentina.
Actinobacteria have frequently been reported in the Andean Puna, including strains of the genus Micrococcus. These strains demonstrate resistance to high levels of UV radiation, arsenic, and multiple antibiotics, and possess large linear plasmids. A comparative analysis of the sequences and putative functions of these plasmids was conducted.
View Article and Find Full Text PDFFirst isolation of from clinical specimens collected on a pig farm in Poland.
J Vet Res
December 2024
Department of Infectious, Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Introduction: Successful retrieval of from porcine clinical specimens has been rarely described, and data has only been obtained from a few swine-producing countries. Therefore, the aim of this study was the isolation of recovered from a specimen originating from a commercial pig farm located in Poland.
Material And Methods: Seven dead 12-week-old pigs weighing 24-26 kg with joint swelling of the hind legs were selected on a modern farrow-to-nursery farm in Poland in October 2023.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!