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Characterization and rheological study of the galactomannan extracted from seeds of Cassia grandis. | LitMetric

Characterization and rheological study of the galactomannan extracted from seeds of Cassia grandis.

Carbohydr Polym

Departamento de Bioquímica/Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco, Avenue Prof. Moraes Rego s/n, Recife CEP: 50.670-420, PE, Brazil. Electronic address:

Published: April 2014

AI Article Synopsis

Article Abstract

Galactomannan extracted from seeds of Cassia grandis with 0.1M NaCl, followed by ethanol precipitation, presented a yield of 36 ± 8%. The polysaccharide has a constant mannose/galactose ratio (2.44:1). Methylation analysis, one and two dimensional NMR spectroscopy confirmed that the polysaccharide has a central core composed of 4-linked β-mannose units, with branches of galactose, linked to the carbohydrate core through α(1-6) linkage. The amorphous nature of the galactomannan was confirmed by X-ray diffraction. Rheological characterization exhibited Newtonian plateaus followed by shear-thinning zones characteristic of polymer solutions up to 1.5% (w/v) and above this value the system exhibited yield stress associated with a weak gel. Adjusting stress-strain curves confirmed a 1.6% (w/v) as the galactomannan concentration value for the sol-gel transition. These results indicate that the galactomannan extracted from C. grandis seeds presents rheological characteristics suitable for applications in pharmaceutical, biomedical, cosmetic and food industries.

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http://dx.doi.org/10.1016/j.carbpol.2014.01.010DOI Listing

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