Aim: Comparative study of the effect of endotoxin tolerance of mice to the effect of lipopolysaccharide (LPS37) and complex of lipopolysaccharide with mice toxin (LPS37-MT) of a virulent Yersinia pestis 231 strain.
Materials And Methods: Preparations of LPS of highly virulent strain Y. pestis 231 obtained by phenol method from cells cultivated at 37 degrees C as well as commercial preparations of S-LPS and R-LPS of Escherichia coli were used. Mice toxin was isolated from vaccine strain Y. pestis EV76. Effect of endotoxin tolerance was determined in mice treated with aminosugar D-galactosamine.
Results: The effect of initial LPS37 and modified form LPS37-MT of Y. pestis 231 was established to significantly differ from each other. When Y. pestis LPS37 is combined with heterologous forms--E. coli LPS or Y. pestis LPS37-MT, the inflammatory response of the organism differs and varies from complete or partial tolerance to complete lack thereof. For LPS37-MT complex only the sequence of administration to bioassay animals of LPS preparations is principal. In the case when primary activation is carried out by LPS37-MT and secondary--by Y. pestis LPS37 or S- and R- forms of E. coli LPS--the tolerance effect is absent. On the contrary, if LPS37-MT is used for recurrent activation against the background of all the other LPS forms including Y. pestis 231 LPS37 the inflammatory response is completely suppressed.
Conclusion: Tolerance of mice to effect of LPS and LPS-MT complex of virulent Y. pestis 231 strain was shown to be different.
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