To evaluate the effects of manure application on continuous maize seed production, 10-year cattle manure on soil properties, heavy metal in soil and plant were evaluated and investigated in calcareous soil. Results showed that manure application increased soil organic matter, total and available nutrients, pH, and electrical conductivity (EC), and the most massive rate caused the highest increase. Manure application led to an increase in exchangeable fraction and an increase of availability of heavy metal. Residual fraction was dominant among all metals, followed by the fraction bound to Fe and Mn oxides. Manure application involved accumulation of heavy metal on corn, but the accumulation in the stem is higher than that in the seed. Manure application led to a high deficiency of total Zn and high accumulation of total Cd in the soil of corn seed production, which should be a risk for safety seed production in calcareous soil in Northwest China.
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